Umbilical artery para-arterial stem cell injection for treating ischemic disease and preparation method thereof

An ischemic disease, stem cell technology, applied in the field of medicine, can solve the problems of low expression of CD146 on the cell surface, weak angiogenesis ability, unclear characteristics, etc., to improve the blood supply of ischemic lower extremities, with significant effect, and promote proliferation. Effect

Active Publication Date: 2019-07-12
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mainly include: (1) The current clinical application is mainly a mixture of umbilical cord Wharton's jelly mesenchymal stem cells / umbilical cord stem cells, the characteristics are not clear, the expression of CD146 on the cell surface is low, and its angiogenesis ability is weak
(2) The rehabilitation of patients with ischemic diseases of the lower limbs depends on adequate blood supply reconstruction, and the application of human umbilical cord Wharton's jelly mesenchymal stem cells in ischemic diseases of the lower limbs is not effective

Method used

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  • Umbilical artery para-arterial stem cell injection for treating ischemic disease and preparation method thereof
  • Umbilical artery para-arterial stem cell injection for treating ischemic disease and preparation method thereof
  • Umbilical artery para-arterial stem cell injection for treating ischemic disease and preparation method thereof

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Embodiment 1

[0026] A para-umbilical cord artery stem cell injection for treating ischemic diseases, comprising suspended para-umbilical cord artery stem cells and platelet-rich umbilical cord plasma. Among them, the content of stem cells adjacent to the umbilical artery is 5×10 6 / mL.

[0027] The preparation method of para-umbilical cord artery stem cell injection for the treatment of ischemic diseases is: para-arterial stem cells are derived from umbilical cord artery tissue culture, and a single para-umbilical cord artery stem cell is obtained by enzymatic digestion, and washed twice with calcium and magnesium-free PBS. Then the cell pellet is resuspended with platelet-rich umbilical cord plasma, and gently mixed to prepare the para-umbilical cord artery stem cell injection. The para-umbilical artery stem cell injection is stored at 4°C for a short time, and used within 12 hours. Shake gently before use.

[0028] Among them, the preparation process of umbilical cord artery stem cells is: t...

Embodiment 2

[0057] An umbilical cord artery stem cell injection for treating ischemic diseases, comprising suspended umbilical cord artery stem cells and PBS for injection containing human serum albumin. Wherein, the content of stem cells adjacent to the umbilical artery is 5×10 6 / mL, the content of human serum albumin is 5% of the volume of PBS.

[0058] The preparation method of para-umbilical cord artery stem cell injection for the treatment of ischemic diseases is: para-arterial stem cells are derived from umbilical cord artery tissue culture, and a single para-umbilical cord artery stem cell is obtained by enzymatic digestion, and washed twice with calcium and magnesium-free PBS. Then the cell pellet was resuspended in PBS containing 5% human serum albumin and mixed gently to prepare the para-umbilical cord artery stem cell injection; the para-umbilical cord artery stem cell injection was stored at 4℃ for a short period of time and used within 12 hours. Shake gently.

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Abstract

The invention discloses an umbilical artery para-aortic stem cell injection for treating an ischemic disease and a preparation method thereof. The injection comprises suspended umbilical artery para-aortic stem cells and a solvent. The solvent may be platelet-rich umbilical cord plasma or PBS for injection containing human serum albumin. The preparation method comprises the steps of after umbilical artery tissue culture, deriving the artery para-arterial stem cells, enzymatically digesting to obtain single umbilical artery para-aortic stem cell, washing twice with PBS, and then gently mixing with the solvent to obtain the umbilical artery para-arterial stem cell injection. The cells are taken from a medical waste umbilical cord and have the advantages of easy separation, rapid proliferation and low immunogenicity. The PBS containing the 5% human serum albumin or the platelet-rich umbilical cord plasma resuspends the umbilical artery para-arterial stem cells to prepare the injection suspension, and the suspension is injected to significantly improve the blood supply of lower limb ischemia model mice.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to an injection, in particular to an umbilical artery stem cell injection for treating ischemic diseases and a preparation method thereof. Background technique [0002] Lower extremity ischemic disease is a common peripheral arterial disease, which refers to the stenosis or occlusion of lower extremity arteries and insufficient blood perfusion caused by various reasons, which leads to intermittent claudication, ulcers, gangrene and other ischemic manifestations of the lower extremities. Class disease. At present, there is no effective treatment and control method for the treatment of ischemic diseases of the lower extremities, and it is not enough to induce enough neovascularization in the extensive ischemic and hypoxic area. Finding high-quality stem cell transplantation to improve the blood perfusion of ischemic lower limbs is an important issue to be solved urgently. [0003] At present, the res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/10A61K35/51A61K35/16A61K35/19A61P9/10C12N5/071
CPCA61K9/0019A61K9/10A61K35/16A61K35/19A61K35/51A61P9/10C12N5/0602C12N2509/00A61K2300/00
Inventor 丁利军孙海翔徐璐李一凡
Owner NANJING DRUM TOWER HOSPITAL
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