Detection kit for predicting attack of early-onset preeclampsia

A detection kit, a technology for preeclampsia, applied in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. The detection method is simple, the specificity and sensitivity are improved, and the effect of a single indicator

Pending Publication Date: 2019-07-12
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0004] Some of the prediction methods reported in previous studies, such as plasma biomarkers and susceptibility genes, cannot be really applied clinically due to their poor specificity, low sensitivity, or the need for multi-molecule joint diagnosis prediction

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  • Detection kit for predicting attack of early-onset preeclampsia
  • Detection kit for predicting attack of early-onset preeclampsia
  • Detection kit for predicting attack of early-onset preeclampsia

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with specific examples, but the examples are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0023] The reagents used in this example are as follows: RNAiso plus reagent for total RNA extraction (manufacturer: Biotech Co., Ltd. (Takara China), product number: 9108), cDNA first strand synthesis reverse transcription reagent (manufacturer: Biotech Technology Co., Ltd. (Takara China), Cat. No.: RR047A), PCR Quantitative Reagent SYBR Green I (manufacturer: Biotech Biotechnology Co., Ltd. (Takara China), Cat. No.: RR420), and primer sequences wer...

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Abstract

The invention provides a detection kit for predicting an attack of early-onset preeclampsia, and discloses a nucleotide sequence shown in SEQ ID NO.1 used for predicting the attack of the early-onsetpreeclampsia, a forward primer sequence shown in SEQ ID NO.2 and a reverse primer sequence shown in SEQ ID NO.3, wherein the forward primer sequence shown in SEQ ID NO.2 and the reverse primer sequence shown in SEQ ID NO.3 are used for the nucleotide sequence. The detection kit can be applied to the clinical experiment operation, a detection method provided by the kit is simple, the index is single, the predicated specificity reaches 100%, the sensitivity reaches 88.9%, and compared with existing detection methods, the specificity and the sensitivity are greatly improved. Therefore, the detection kit has a wide application prospect in an aspect of predicting the attack of the early-onset preeclampsia.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a detection kit for predicting the onset of early-onset preeclampsia. Background technique [0002] Early-onset preeclampsia is a serious disease during pregnancy, usually onset after 20 weeks of gestation, and characterized by persistently elevated blood pressure (≥160 / 110 mmHg). It can lead to complications such as postpartum hemorrhage, placental abruption, premature birth, and stillbirth in pregnant women, often causing serious maternal and infant morbidity and mortality. The incidence of early-onset preeclampsia in my country is about 3%, and the incidence of preeclampsia in my country is about 2-8%. Therefore, if it can be predicted early and intervened in a timely manner, the morbidity and mortality of mothers and infants will be greatly reduced. [0003] At present, the clinical prediction of early-onset preeclampsia still relies on physicians' screening of blood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/158
Inventor 张鹏杰孙淼徐智策陈雄辉张跃明李凌君周秀文
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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