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Kit for determining prognosis of colorectal cancer through protein marker

A protein marker, colorectal cancer technology, applied in the field of kits for judging the prognosis of colorectal cancer, to achieve the best benefit effect

Active Publication Date: 2019-07-16
成都市第三人民医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, no index has been evaluated with absolute consistency by global scholars, which may be related to the diversification of detection methods and the heterogeneity of tumors

Method used

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  • Kit for determining prognosis of colorectal cancer through protein marker
  • Kit for determining prognosis of colorectal cancer through protein marker
  • Kit for determining prognosis of colorectal cancer through protein marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0049] For the tumor tissue samples of 130 patients with colorectal cancer, IHC method was used to detect the level of Osteoglycin protein, combined with the five-year overall survival of the patients, univariate and multivariate analysis of variance were performed.

[0050] (1) Preparation of samples:

[0051] The isolated surgical tissue within 30 minutes was put into neutral formalin fixative and fixed overnight, rinsed with running water, dehydrated, transparent and soaked in wax to prepare tissue wax blocks. Cases of stage II colon cancer judged by histopathology. Each selected 3 typical cancer tissue spots and 2 normal paracancerous tissue spots to make tissue microarray and prepare 4 μm slices.

[0052] (2) IHC: The specific detection method of protein is as follows:

[0053] Place the front of the tissue chip facing the wind in a 65°C oven and bake for 40 minutes to make the specimen adhere better, then immerse in xylene washing tanks I, II, and III, place them in a ...

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Abstract

The invention relates to the use of a protein marker detection ligand in preparation of a product for determining prognosis of a colorectal cancer and a kit for determining the prognosis of the colorectal cancer. The ligand is used to be combined with an Osteoglycin protein in a detected sample, the prognosis of a patient with the colorectal cancer can be effectively judged, postoperative medication of the patient with the colorectal cancer is guided, and the patient can gain the best benefits.

Description

technical field [0001] The disclosure relates to the technical fields of molecular biology and clinical detection, and in particular, relates to the use of protein markers in products for judging the prognosis of colorectal cancer and a kit for judging the prognosis of colorectal cancer through protein markers. Background technique [0002] Colon cancer is one of the most common digestive system tumors, and the mortality rate of colorectal cancer ranks fourth among all tumors worldwide. According to the latest data provided by the National Cancer Prevention and Control Office of the Ministry of Health, colorectal cancer ranks third and fifth in the incidence and mortality of malignant tumors in my country, and its incidence rate has reached the second in urban areas. Most of the deaths from colon cancer are caused by recurrence and metastasis. So far, the molecular mechanism related to the recurrence and metastasis of colorectal cancer has not been elucidated. Finding closel...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N33/577
CPCG01N33/6893G01N33/57446G01N33/577G01N2800/065
Inventor 张彤彤郭元彪赵聪周政包勇包郁
Owner 成都市第三人民医院
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