Durable enzyme-based biosensor and process for drop deposition immobilization
A sensor and sensor element technology, applied in the direction of sensors, nano-scale sensors, instruments, etc., can solve the problems of limited use, short shelf life, etc.
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[0080] The technology described herein involves the detection of biomarkers using biomolecule-based biosensors that have an extended shelf life compared to previous biosensors. In preferred embodiments, the biosensor relies on the use of enzymes that catalyze electron transfer reactions specific for the biomarkers to be detected. In other embodiments, biomarkers are detected using biomolecules other than enzymes (eg, non-enzymatic proteins, antibodies, aptamers, lipids, nucleotides, bacteria, and any combination thereof).
[0081] Previous enzyme-based biosensors (such as those using glucose oxidase) often suffer from a short shelf life, often less than a month, or only a few days, mainly because the enzymes used in their design are unstable. . This limits the clinical use of enzyme-based biosensors. See Gibson, T.D. Biosensors: The Stability Problem, Analusis, 1999, 27(7): p.630-638. Biosensors fabricated according to the present technique overcome this shortcoming. This ...
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