Durable enzyme-based biosensor and process for drop deposition immobilization

A sensor and sensor element technology, applied in the direction of sensors, nano-scale sensors, instruments, etc., can solve the problems of limited use, short shelf life, etc.

Active Publication Date: 2019-07-16
NORTHEASTERN UNIV
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  • Abstract
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Problems solved by technology

However, many of these biosensors that use enzymes to detect analytes have a short sh

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  • Durable enzyme-based biosensor and process for drop deposition immobilization
  • Durable enzyme-based biosensor and process for drop deposition immobilization
  • Durable enzyme-based biosensor and process for drop deposition immobilization

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Embodiment Construction

[0080] The technology described herein involves the detection of biomarkers using biomolecule-based biosensors that have an extended shelf life compared to previous biosensors. In preferred embodiments, the biosensor relies on the use of enzymes that catalyze electron transfer reactions specific for the biomarkers to be detected. In other embodiments, biomarkers are detected using biomolecules other than enzymes (eg, non-enzymatic proteins, antibodies, aptamers, lipids, nucleotides, bacteria, and any combination thereof).

[0081] Previous enzyme-based biosensors (such as those using glucose oxidase) often suffer from a short shelf life, often less than a month, or only a few days, mainly because the enzymes used in their design are unstable. . This limits the clinical use of enzyme-based biosensors. See Gibson, T.D. Biosensors: The Stability Problem, Analusis, 1999, 27(7): p.630-638. Biosensors fabricated according to the present technique overcome this shortcoming. This ...

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Abstract

A sensor having improved shelf life is provided for determining concentration of a biomarker in a liquid sample. The sensor functions by electrochemical detection and requires use of a bio-molecule, e.g., an enzyme that catalyzes an electron transfer reaction specific for the biomarker. The sensor requires use of a quaternary ammonium compound as a bio-linker. It further requires crosslinking of the bio-molecule for improved stability.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Application No. 62 / 432,513, entitled "Durable Enzyme-Based Biosensor Platform Provided by Droplet Deposition Immobilization Method," filed December 9, 2016, the entire contents of which Incorporated herein by reference. Background technique [0003] Successful health care systems provide better health to more people on an ongoing basis at lower cost. Real-time point-of-care (POC) testing can aid in effective disease control and appropriate therapeutic intervention. In this regard, efforts are underway to develop non-invasive methods of screening cancer patients using saliva as an indicator fluid. Wong, J Calif Dent Assoc, 2006, 2006.34(4): p.303- [0004] 8; Wong, J Am Dent Assoc, 2006, 137(3): p.313-21; and Castagnola et al., [0005] Acta Otorhinolaryngologica Italica, 2011, 31(6): p.347-357. In the field of chronic disease management, biomarker monitoring techn...

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Application Information

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IPC IPC(8): G01N27/327A61B5/145A61B5/1468A61B5/1486C09D5/16C09D7/40
CPCA61B5/1486G01N27/3271G01N27/3278A61B5/14532A61B2562/0285
Inventor Y·杜M·L·王
Owner NORTHEASTERN UNIV
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