Quantitative detection method for vomitoxin in flour

Abstract

The invention discloses a quantitative detection method for vomitoxin in flour. Through an enrichment technology, a high-concentration DON (deoxynivalenol) flour sample is prepared, then, a near infrared spectrum technology is adopted to measure the DON in the high-concentration DON flour sample, and finally, the DON is converted into the DON content of an original flour sample. Through the research, a separated enrichment technology is adopted to improve DON concentration, then, a DON quantitative analysis model based on a BP (Back Propagation) neural network is established, a relevant coefficient is above 0.92, and a modeling effect is good. The R of a neural network model is greatly improved, RMSEP (Root Mean Square Error Of Prediction) is lowered to 0.273ppm from 0.426, and therefore,the stability and the adaption of the model are further improved. A separated and enriched flour prediction set is adopted, so that an error mean square root is relatively small so as to illustrate that a difference with a true value is small. When a large flour factory detects the DON content, a method for directly scanning a flour spectrum can be adopted for carrying out factory leaving measurement, and a preliminary screening and detecting method is provided.

Description

technical field [0001] The invention relates to a quantitative detection method of vomitoxin in flour. Background technique [0002] Wheat is one of the main food crops in the world. Flour, as the main processed product of wheat, occupies a large proportion in people's diet structure. However, wheat will produce vomitoxin, also known as deoxygenation, when it is affected by head blight during its own growth. The toxic compound nivalenol (DON). Deoxynivalenol is named for its ability to cause vomiting in humans and animals. Acute poisoning symptoms such as vomiting, fever, anorexia, and diarrhea will occur when people overeat food contaminated by DON. Therefore, my country stipulates that the concentration of DON in flour products should be lower than 1ppm. [0003] At present, the methods for the determination of DON mainly include: liquid chromatography-tandem mass spectrometry; immunoaffinity chromatography purification high-performance liquid chromatography; thin-layer...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that when the content of vomitoxin in flour is lower than 5ppm, it is very difficult to use near-infrared spectroscopy to quantitatively analyze it, and the detection error is relatively large. Flour samples, and then use near-infrared spectroscopy to measure the DON in the high-concentration DON flour sample, and finally convert it into the DON content of the original flour sample

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