Determination method of trace elements in milk powder
A technology of medium and trace elements and detection methods, which is applied in the field of detection of trace elements in milk powder, can solve the problem that the detection method needs to be studied and so on.
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Embodiment 1
[0050] Accurately weigh 0.5g (accurate to 0.001g) of the sample of infant formula milk powder (I) into a 50mL centrifuge tube, add 25mL of 5% nitric acid solution by volume, tighten the lid and vortex to mix evenly to fully dissolve the sample. Add 1.5 mL of perchloric acid to the tube with the lid (the final concentration of perchloric acid is 6% by mass), then vortex to mix evenly, and then sonicate for 5 min in an ultrasonic cleaner. Centrifuge at 8000 rpm for 10 min. After the centrifugation, carefully remove the sample and avoid redissolving the protein precipitate into the solution. Use a 20mL syringe (with a needle) to carefully draw the clear solution in the lower layer, and filter it through a 0.45 μm water-based filter to obtain a clear solution of the sample. At the same time, a reagent blank experiment was performed.
[0051] The test results of the samples are shown in Table 1. It can be seen that the precision of the detection results of each element in infant f...
Embodiment 2
[0055] Accurately weigh 0.5g (accurate to 0.001g) of the infant formula milk powder (Ⅰ) sample in a 50mL centrifuge tube, add the standard working solution of each element to prepare a spiked sample infant formula milk powder (Ⅱ), add 5% by volume accurately 25mL of nitric acid solution, tighten the lid and vortex to mix well, so that the sample is fully dissolved and completely, open the lid and add 1.5mL of perchloric acid to the tube (the final concentration of perchloric acid is 6% by mass), then vortex to mix evenly, and then put in Ultrasonic in an ultrasonic cleaner for 5 min. After the ultrasonication is over, take out the centrifuge tube and centrifuge at 8000 rpm for 10 min in a high-speed centrifuge. After the centrifugation is completed, carefully remove the sample and avoid redissolving the protein precipitate in the solution. Use a 20mL syringe (with a needle) to carefully draw the clear solution in the lower layer, and filter it through a 0.45 μm water-based filt...
Embodiment 3
[0061] Accurately weigh 0.5g (accurate to 0.001g) of the sample of infant formula milk powder (Ⅰ) into a 50mL centrifuge tube, add the standard working solution of each element to prepare a spiked sample infant formula milk powder (Ⅲ), and add 5% by volume accurately. 25mL of nitric acid solution, tighten the lid and vortex to mix well, so that the sample is fully dissolved and completely, open the lid and add 1.5mL of perchloric acid to the tube (the final concentration of perchloric acid is 6% by mass), then vortex to mix evenly, and then put in Ultrasonic in an ultrasonic cleaner for 5 min. After the ultrasonication is over, take out the centrifuge tube and centrifuge at 8000 rpm for 10 min in a high-speed centrifuge. After the centrifugation is completed, carefully remove the sample and avoid redissolving the protein precipitate in the solution. Use a 20mL syringe (with a needle) to carefully draw the clear solution in the lower layer, and filter it through a 0.45 μm water-...
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