APRR2 gene related to green peel traits of melons

A peel and melon technology, applied in genetic engineering, plant genetic improvement, DNA/RNA fragments, etc., can solve problems such as not being located

Pending Publication Date: 2019-08-09
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the genes that control the color of other melon skins have not been mapped so far

Method used

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  • APRR2 gene related to green peel traits of melons
  • APRR2 gene related to green peel traits of melons
  • APRR2 gene related to green peel traits of melons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] (1) Preliminary study on genetic phenotype

[0042] Parents B432 (yellow skin) and B421 (green skin) and their F 2 The skin color traits of each individual plant of the population materials were investigated, recorded and photographed.

[0043] Statistical analysis was carried out on the skin color traits of all muskmelon materials, and the results showed that:

[0044] Among the BSA-seq test materials: the skin color of the female parent B432 is yellow, the male parent B421 is green, and the skin color of the F 1 The skin color performance is consistent, all of which are green; while BC 1 The skin color of the offspring of P1 was separated, among which there were 42 plants with green skin, 15 plants with white skin, and 21 plants with yellow skin. After chi-square test, χ 2 =1.39, less than χ 2 0.05,2 =5.99, indicating BC 1 The skin color separation ratio of P1 offspring conforms to the theoretical value (green skin: white skin: yellow skin = 2:1:1);

[0045] Fu...

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Abstract

The invention belongs to the technical field of melon gene breeding development and particularly relates to an APRR2 gene related to green peel traits of melons. The gene is 1134bp in length and related to the green character of melon peel, and a base sequence is shown as SEQ ID NO.1. In addition, the invention also provides an indel molecular marker for detecting the APRR2 gene. On the basis of asix-generation genetic population constructed by yellow peel and green peel materials, the genetic law of melon peel colors is preliminarily analyzed, and finally the gene closely related to the formation of melon green peel is determined. Furthermore, it is found that a 13 bp basic group insertion of the gene exists in white and yellow melon peel materials, and an indel marker is designed on thebasis. In conclusion, the molecular marker can be used for early selection in melon peel color breeding, and on the basis of the related research results of the application, a certain foundation canbe laid for the breeding of new melon varieties.

Description

technical field [0001] The application belongs to the technical field of muskmelon gene breeding and development, and specifically relates to an APRR2 gene related to the green peel trait of muskmelon. Background technique [0002] melon( Cucusis melon L.) is a Cucurbitaceae ( Cucurbitaceae ) Cucumber is an annual vine herb. China has a long history of melon cultivation and is also the world's largest producer and consumer of melons. According to FAO statistics, in 2016 my country's melon planting area reached 474,700 hm 2 , the output accounted for 51.16% of the world's total output. Melon is a species with very rich genetic diversity, such as skin color, fruit shape, fruit size. Fruit skin color is not only an important appearance quality of melon fruit, but also the most intuitive evaluation standard that affects consumers' choice. Therefore, selecting muskmelon varieties that meet the needs of various markets and have diverse skin colors is also an important part ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12Q1/6895C12Q1/6858C12N15/11
CPCC07K14/415C12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 赵光伟徐永阳贺玉花林涛练群黄三文欧点点王平勇徐志红孔维虎张健
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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