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Construction Method of Peanut Genetic Population

A technology for genetic populations and construction methods, used in biochemical equipment and methods, botanical equipment and methods, and microbial assay/inspection, etc., and can solve problems such as low polymorphism, low efficiency, and cumbersome screening work.

Active Publication Date: 2022-04-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of these methods first requires polymorphism screening of the hybrid parents, and the hybrid parents widely used in production have a close relationship and low polymorphism, which makes the screening work cumbersome and inefficient.

Method used

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  • Construction Method of Peanut Genetic Population
  • Construction Method of Peanut Genetic Population
  • Construction Method of Peanut Genetic Population

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Parents: Peanut high-oil parent Yuhua 14, FAD2 loci are AhFAD2A and AhFAD2B (genotype is AABB); peanut low-oil parent SPI098, FAD2 loci are AhFAD2a and AhFAD2b (genotype is aabb), both FAD2 loci genotype differences such as figure 1 and figure 2 As shown, there is a significant difference in oil content between Yuhua 14 and SPI098, as shown in image 3 shown.

[0046] The construction method of peanut genetic population, the steps are as follows:

[0047] 1. Artificial pollination hybridization of peanuts

[0048] The high-oil parent Yuhua 14 (common oleic acid content) and the low-oil parent SPI098 (high oleic acid content) were planted at intervals in the field. One week before the full flowering period (about 7 weeks after sowing), the flower buds of the female parent Yuhua 14 were manually removed for about 1 week; the anthers in the female parent flower buds were removed with tweezers after 17:00 every afternoon in the full flowering period, and the detasselin...

Embodiment 2

[0068] Parents: large peanut variety Luhua 11, FAD2 loci of AhFAD2a and AhFAD2B (genotype aaBB), peanut small fruit line BC54 crossed between Luhua 11 and Kainong 1715, then backcrossed with female parent Luhua 114 The FAD2 locus is AhFAD2a and AhFAD2b (genotype is aabb), and the genotype difference of the two FAD2 loci is as follows Figure 7 and figure 2 As shown, there is a significant difference in pod length between Luhua 11 and BC54, such as Figure 8 shown.

[0069] A method for constructing a peanut genetic population, the steps are as follows:

[0070] 1. Artificial pollination hybridization of peanuts

[0071] The large-fruit parent Luhua 11 (common oleic acid content) and the small-fruit parent Luhua 11 and Kainong 1715 backcross progeny BC54 (high oleic acid content) were planted at intervals in the field. One week before the full flowering period (about 7 weeks after sowing), the flower buds of the female parent Luhua 11 were manually removed for about one we...

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Abstract

The invention discloses a method for constructing a peanut genetic population, and belongs to the technical field of genetic population construction. The method for constructing the peanut genetic population of the present invention is to use two parental peanuts with FAD2 genotype differences to carry out hybridization, and use competitive allele-specific PCR (Kompetitive Allele Specific PCR, KASP) to detect F 1 The FAD2 genotype of the substitute seed kernel, the genotype of FAD2 is screened as heterozygous kernel, and the genotype of FAD2 is removed as homozygous kernel; observation and detection of FAD2 1 Single Seed Harvesting F 2 Whether the target phenotype of generation segregates, for F 2 Lines with indistinct phenotype segregation were tested for FAD2 genotype to eliminate homozygous lines, and then related genetic populations were constructed by selfing or backcrossing. The method of the invention can simply and efficiently screen true hybrids, and construct effective segregation populations with target traits.

Description

technical field [0001] The invention belongs to the technical field of genetic population construction, and in particular relates to a method for constructing a peanut genetic population. Background technique [0002] Sexual hybridization is an important approach in crop breeding and an essential method for genetic population construction. For self-pollinated crops, artificial hybridization needs to go through two stages of emasculation and artificial pollination. During peanut hybridization, non-pollinated flowers need to be removed before and after hybridization to avoid mixing of non-hybrid fruit needles. However, in the actual operation process, due to incomplete removal of female stamens and omission of defloration, there will be a certain proportion of false hybrids in the harvested hybrid fruits. Therefore, screening true hybrids and removing false hybrids has become an important task after the hybrid fruit is harvested. For a long time, breeders have observed F 1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02C12Q1/6858C12Q1/6895
CPCA01H1/02A01H1/022C12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2563/107
Inventor 乔利仙唐艳艳邱晓臣纪红昌胡畅丽刘文平朱虹隋炯明张晓军王晶珊
Owner QINGDAO AGRI UNIV
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