Immunoadsorbent for purifying aflatoxin, sterigmatocystin and cyclopiazonic acid, and composite affinity column
A variegated aspergillus and aflatoxin technology, which is applied in the directions of solid adsorbent liquid separation, selective adsorption, chemical instruments and methods, etc. and other problems to achieve stable performance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] Example 1: Obtaining of Universal Monoclonal Antibody to Aflatoxin
[0032] The universal anti-aflatoxin monoclonal antibody is produced by the secretion of the hybridoma cell line 1C11 with the preservation number CCTCC NO.C201013, and is specifically prepared in advance according to the method reported in the patent application number 201010245095.5. The preparation method is: the obtained hybridoma cells Strain 1C11 was injected intraperitoneally into BALB / c mice treated with incomplete Freund's adjuvant in advance, and the ascites of the mice was collected, purified and treated to obtain a universal anti-aflatoxin monoclonal antibody. Wherein, the purification method is octanoic acid-ammonium sulfate method, and the specific operation is as follows: the ascites is taken out from the -20°C refrigerator and thawed at room temperature. Filter the mouse ascites with double-layer filter paper, centrifuge the filtered ascites at 4°C, 12000r / min for more than 15min, absorb...
Embodiment 2
[0034] Embodiment 2: Obtaining of anti-aspergillus versicolor monoclonal antibody
[0035]The anti-aspergillus versicolor monoclonal antibody is produced by the secretion of the hybridoma cell line 1C11 with the deposit number CCTCC NO.C2013187, and it is specifically prepared in advance according to the method reported in the patent application number 201410115952.8. The preparation method is: the obtained hybridoma cells Strain ST03 was intraperitoneally injected into BALB / c mice treated with Freund's incomplete adjuvant in advance, and the ascites of the mice was collected, purified and treated to obtain anti-Varistoxin monoclonal antibodies. Wherein, the purification method is octanoic acid-ammonium sulfate method, and the specific operation is as follows: the ascites is taken out from the -20°C refrigerator and thawed at room temperature. Filter the mouse ascites with double-layer filter paper, centrifuge the filtered ascites at 4°C, 12000r / min for more than 15min, absorb...
Embodiment 3
[0037] Example 3: Obtaining of Anti-Cyclopiazonic Acid Monoclonal Antibody
[0038] The anti-cyclopianic acid monoclonal antibody is produced by the hybridoma cell line YTT-2 with the preservation number CCTCC NO.C C201871. details as follows:
[0039] Cyclopiazonic acid monoclonal antibody hybridoma cell line YTT-2 was intraperitoneally injected into BALB / c mice treated with incomplete Freund's adjuvant in advance, the ascites of the mice was collected, and the antibody was purified by octanoic acid-ammonium sulfate method , the specific operation is: filter mouse ascites with double-layer filter paper, centrifuge the filtered ascites at 4°C, 12000r / min for more than 15min, absorb the supernatant, mix the supernatant with 4 times the volume of acetate buffer, and stir While slowly adding n-octanoic acid, the volume of n-octanoic acid required per milliliter of ascites is 30-35 μL, mix at room temperature for 30-60 min, let stand at 4°C for more than 2 h, then centrifuge at 1...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More