Biomarkers of oral, pharyngeal and laryngeal cancers

A technique for oral cancer and oral cavity, applied in the field of head and neck cancer

Pending Publication Date: 2019-08-16
N 特兰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No clinically available biomarkers enable early detection of these cancers

Method used

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  • Biomarkers of oral, pharyngeal and laryngeal cancers
  • Biomarkers of oral, pharyngeal and laryngeal cancers
  • Biomarkers of oral, pharyngeal and laryngeal cancers

Examples

Experimental program
Comparison scheme
Effect test

example

[0137] general method

[0138] Head and neck squamous cell carcinoma (HNSCC) serum samples were obtained from subjects with informed consent, approved by the ethics committees of the University of Technology and the Royal Prince Alfred Hospital. Serum samples were selected from pathologically diagnosed oral cavity tumors (n=42; mean age 62.1 years; 68% males), oropharyngeal tumors (n=5; mean age 71.4 years; 60% males), or pharynx / larynx tumors 52 HNSCC patients (n=5; mean age 60.8 years; 80% male). Details are provided in Table 3 below.

[0139] table 3

[0140]

[0141]

[0142] Sera were also collected from 11 healthy (HNSCC-free) subjects (mean age 55.7 years; 63% male) to serve as controls for this study (see also Table 3 above).

[0143] From each subject, 5 mL of blood was collected directly into the BD in the blood collection tube. All samples were collected at room temperature (before surgery in the case of HNSCC patients) and BD by centrifugation at 800rpm...

example 1

[0151] Example 1 - Differential miRNA expression between HNSCC and tumor-free controls

[0152] The inventors first investigated miRNA expression in compiled pooled HNSCC serum samples (n=52) compared to tumor-free control serum samples (n=11). Volcano plot analysis identified 53 miRNAs that were significantly overexpressed (upregulated) and 40 miRNAs that were significantly underexpressed (downregulated) in sera from HNSCC subjects compared with sera from tumor-free control subjects.

[0153] figure 1 Shown are the ten most overexpressed and ten least expressed miRNAs with p-values ​​less than 0.000001 in sera from HNSCC subjects compared to sera from tumor-free control subjects, as determined from volcano plot analysis. Table 4 presents the figure 1 p-values ​​and fold changes for the six most overexpressed and three least expressed miRNAs, and the number of known gene targets for each miRNA.

[0154] Table 4

[0155]

example 2

[0156] Example 2 - Differential miRNA expression between oral tumor group and tumor-free control group

[0157] The inventors then investigated miRNA expression in oral tumor pooled serum samples (n=42) and tumor-free control samples (n=11). Volcano plot analysis identified 130 miRNAs that were significantly overexpressed (upregulated) and significantly underexpressed (downregulated) in sera from subjects with oral tumors compared with sera from tumor-free control subjects. 36 miRNAs.

[0158] figure 2 Shown are the ten most overexpressed and ten least expressed miRNAs with a p-value less than 0.000001 in sera from subjects with oral cavity tumors compared to sera from tumor-free control subjects, as analyzed from the volcano plot determined. Most of the differentially expressed miRNAs were found to be overexpressed (increased between 100 and 1000 fold). The lowest expressed miRNA (miR-129-3p) exhibited a relatively modest 100-fold decrease in expression.

[0159] Gene o...

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Abstract

Provided herein are methods for detecting a head and neck cancer of the oral cavity or throat, optionally oral squamous cell carcinoma, comprising executing the step of determining the expression of two or more miRNA in a biological sample obtained from a subject, wherein the two or more miRNA are selected from the group consisting of hsa-let-7a, hsa-miR-16, hsa-miR-21, hsa-miR-451, hsa-miR-486-5pand hsa-miR-92a- 3p, and wherein the level of expression of said two or more niiRNAs in the biological sample relative to the level of expression of said two or more miRNAs in one or more cancer-freereference samples is indicative of the presence of a head and neck cancer of the oral cavity or throat of the subject.

Description

technical field [0001] The present disclosure relates generally to head and neck cancers for the oral cavity or throat, particularly oral cavity cancer, pharyngeal cancer, and laryngeal cancer, and more particularly oral squamous cell carcinoma, oropharyngeal squamous cell carcinoma, pharyngeal squamous cell carcinoma, and laryngeal squamous cell carcinoma. Methods and protocols for the diagnosis and prognosis of squamous cell carcinoma. Background technique [0002] Despite advances in our understanding and therapeutic treatment of many forms of cancer, cancer remains one of the leading causes of death worldwide, and the prevalence of many cancers is increasing. Cancers of the oral cavity, pharynx, and larynx affect organs that are critical to basic functions such as speech and swallowing, and often have a major impact on quality of life. They generally have a poor prognosis and are associated with significant morbidity, with approximately 43% of patients surviving no more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q2600/178C12Q1/6886C12Q2600/118C12Q1/68C12Q2600/106C12Q2600/112C12Q2600/158
Inventor N·特兰S·库利
Owner N 特兰
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