Yew branch and leaf extract, extraction method and applications thereof
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A technology of yew branches and leaves, and an extraction method, which is applied in the field of yew branches and leaves extracts, and can solve problems such as unseen problems.
Inactive Publication Date: 2013-03-06
JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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[0003] At present, there are no related reports about the application of yew branches and leaves extracts in the preparation of drugs for the treatment of anal squamous cell carcinoma, esophageal squamous cell carcinoma, tonsil squamous cell carcinoma, etc. In view of this, the present invention is proposed
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Embodiment 1
[0028] (1) Preparation of extract 1: take yew branches, dip in ethanol solution with a mass concentration of 70%-75%, extract with ethanol solution, continue to extract the remaining medicinal residues with water, combine water and ethanol solution, and reflux with petroleum ether , Soxhlet extraction to recover the solvent to obtain the extract Z-1;
[0029] Or yew leaves, the operation steps are the same as yew branches, and the corresponding extract is Y-1;
[0030] (2) Gradient elution: in terms of mass concentration: dissolve the prepared extract in water, apply HP20 macroporous resin, and follow the sequence of water solution, 20% alcohol solution, 40% alcohol solution, 60% alcohol solution, 80% % alcohol solution, 95% alcohol solution and acetone solution gradient elution, the eluents obtained in sequence are Z-2, Z-3, Z-4, Z-5, Z-6, and 95% ethanol and Acetone eluent to obtain eluents Z-7 and Z-8, which are yew branch extracts; the eluents are 9 column volumes, and th...
Embodiment 2
[0034] Compared with Example 1, the only difference is that in this example, the branches and leaves of Taxus chinensis were mixed and extracted at the same time, the eluents were all 8 times the column volume, and the flow rate was 1 mL / min.
Embodiment 3
[0036] Compared with Example 1, the only difference is that in this example, only the leaves of Taxus chinensis were extracted, the eluents were all 10 times the column volume, and the flow rate was 1.5mL / min.
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Abstract
The present invention relates to a yew branch and leaf extract, an extraction method and applications thereof. The yew branch and leaf extract is extracted by adopting branches and leaves of Taxus chinensis var. mairei as raw materials, and comprises cephalomannine and 7-epitaxol, wherein the active ingredients provide an inhabitation effect for squamous cells in skin cancers, and especially provide significant treatment effects for anal squamous cell carcinoma, esophagus squamous carcinoma and tonsil squamous cell carcinoma. According to the present invention, resource advantages of the yew are utilized to deep develop, utilize and research the Taxus chinensis var. mairei; and a yew anti-skin cancer component extraction method with a characteristic of simple operation is provided, and an effect of the component in anti-skin cancer application is determined.
Description
technical field [0001] The invention relates to an extract of yew branches and leaves, its extraction method and application, and the extracted active ingredients are used for the treatment of skin cancer. Background technique [0002] Taxol extracted from the bark and branches and leaves of Taxus chinensis is a natural compound recognized by the world as having anti-cancer effects. Taxus chinensis is a tree species with high economic value integrating ornamental, timber and medicinal uses. So far, chemists have extracted more than 300 taxane diterpenoids and many alkaloids from this genus, some of which, such as taxidine, also have anticancer activity similar to paclitaxel, and other Some components such as 1-hydroxybaccatin I can be chemically modified to synthesize paclitaxel. In addition, some scholars have isolated 1 ecdysterone, 5 triterpenes, 7 lignans, 3 glycosides, 8 biflavones, and flavonoid kaempferol-4 from this genus. - 14 kinds of methyl ether, other organic ...
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