Medicinal composition against bovine pasteurella multocida

A Pasteurella, multi-kill technology, used in antibacterial drugs, pharmaceutical formulations, organic active ingredients, etc., can solve problems such as drug resistance, and achieve the effects of reducing toxic side effects, efficient bactericidal activity, and good antibacterial effects

Inactive Publication Date: 2019-08-20
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the treatment of bovine pasteurellosis mainly relies on antibiotics, but with the long-term use of antibiotics, the problem of drug resistance inevitably appears

Method used

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  • Medicinal composition against bovine pasteurella multocida
  • Medicinal composition against bovine pasteurella multocida
  • Medicinal composition against bovine pasteurella multocida

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the combined antibacterial test of pharmaceutical composition to Pasteurella multocida

[0021] 1. Experimental materials and methods

[0022] Reagents and reagents:

[0023] Ciprofloxacin (Ciprofloxacin), Thioridazine (Thioridazine) and Tetracycline (Tetracyclines) were purchased from MCE Company, with purity >98.74%, >99.93%, and >98%, respectively.

[0024] Indicator strain:

[0025] Escherichia coli: Escherichia coli, 25922 (E. coli 25922); standard strain 450, clinically isolated Pa strains: Pa 01, Pa 02, Pa 03, Pa 04, Pa 05, Pa 06, Pa 07, Pa 08, Pa09, Pa 010.

[0026] Medium:

[0027] BHI medium: 10.0g peptone, 12.5g dehydrated calf brain extract powder, 5g dehydrated beef heart extract powder, 5g sodium chloride, 2g glucose, 2.5g disodium hydrogen phosphate in 1000mL distilled water, and adjust the pH to 7.4. The prepared culture medium was autoclaved at 121°C, and after 20 minutes, it was ready for use.

[0028] MH medium: mix 2.0 g of b...

Embodiment 2

[0050] Embodiment 2: The effect of the drug composition administration treatment on the Pneumonia model of Pasteurella multocida bovine

[0051] 1. Experimental materials and methods

[0052] Reagents and reagents:

[0053] Ciprofloxacin (Ciprofloxacin), Thioridazine (Thioridazine) and Tetracycline (Tetracyclines) were purchased from MCE Company, with purity >98.74%, >99.93%, and >98%, respectively.

[0054] Strains:

[0055] Standard Pa strain 450, clinical isolate Pa strain Pa 1.

[0056] Medium:

[0057] BHI medium: 10.0g peptone, 12.5g dehydrated calf brain extract powder, 5g dehydrated beef heart extract powder, 5g sodium chloride, 2g glucose, 2.5g disodium hydrogen phosphate in 1000mL distilled water, and adjust the pH to 7.4. The prepared culture medium was autoclaved at 121°C, and after 20 minutes, it was ready for use.

[0058] animal

[0059] SPF-grade BALB / C mice, male, weighing 18-22g, were purchased from Changchun Yisi Experimental Animal Co., Ltd. The lab...

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Abstract

The invention provides a medicinal composition against bovine pasteurella multocida, belongs to the technical field of medicinal compositions, and aims to provide an anti-bacterial medicinal composition for treating bovine pasteurella multocida infection by effectively synthesizing a combined medicine from three medicinal materials. The effective ingredients of the medicinal composition are ciprofloxacin, thioridazine and tetracycline. The medicinal composition has clinical effects including bacterium resistance, inflammation diminishing and the like, and can effectively treat bovine pasteurella multocida infection. Furthermore, the medicinal composition has efficient bactericidal activity and can greatly reduce dosage of administration, so that toxic and side effects of medicines can be greatly reduced. According to the fundamental mechanism of the medicinal composition effect, an excellent antibacterial effect can be achieved through an efflux pump for jointly inhibiting bacteria andsynthesis and duplication of DNA. The clinical curative effect and safety of the medicine can be further proved.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical compositions. Background technique [0002] Pasteurella is a sporeless, non-motile, facultative anaerobic, Gram-negative small bacillus that often stains heavily at both ends of the bacteria. The identified species of this genus are Pasteurella multocida, Pasteurella pneumophila, Pasteurella hemolyticus, Pasteurella urinae and Pasteurella anatipestifer. Pasteurella multocida is a two-segment blunt round, central micro-protruding brevibacterium or coccus, non-spore-forming, non-motile, flagella-free, Gram-negative aerobic facultative anaerobic bacteria. [0003] Pasteurellosis is a zoonotic infectious disease mainly caused by pathogenic bacteria such as Pasteurella multocida. Pasteurella can be transmitted among various animals, and animal bites can also directly cause pathogenic bacteria to enter the epithelial cells of the human body, causing local tissue damage and even systemic disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/496A61K31/65A61K31/5415A61P31/04
CPCA61K31/496A61K31/5415A61K31/65A61P31/04A61K2300/00
Inventor 于录安雅男王雪飞栾文静王超徐红月
Owner JILIN UNIV
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