Storage device for mesenchymal stem cell exosomes

Abstract

The invention discloses a storage device for mesenchymal stem cell exosomes. A low-temperature box with the internal temperature kept at -20 DEG C is included, and is internally provided with a rotatable rotating base; eight storing cavities are formed in the rotating base in the circumferential direction in an array manner; test tube frames capable of sliding are arranged in the storing cavities;test tubes containing the exosomes can be placed in the test tube frames; and stabilizing devices for elastically fixing the test tubes are arranged in the test tube frames. The storage device for the mesenchymal stem cell exosomes is simple in structure, convenient to operate, and convenient to maintain; and the device can be used for effectively keeping the temperature of the test tubes, to-be-taken test tubes can be independently taken out in the taking and using process, temperature changing of other test tubes is avoided, the storing effect of the test tubes is guaranteed, and thus the device has high using and popularizing value.

Description

technical field [0001] The invention relates to the field of cell exosome storage, in particular to a storage device for mesenchymal stem cell exosomes. Background technique [0002] At present, a large number of studies have shown that exosomes derived from mesenchymal stem cells can be transferred between cells through their protein and RNA components, and play a role in tissue repair, immunosuppression, and immune regulation similar to mesenchymal stem cells. Body research is expected to become a key research direction in the field of biomedicine in the future. Since the exosome membrane is rich in sphingomyelin, cholesterol, ceramide and lipid raft domains, it can resist the dissolution of non-ionic detergents at low temperatures and protect the contents from degradation. Therefore, when operating exosomes, try to operate in a low-temperature environment and reduce the number of repeated freezing and thawing. Exosomes can be stored at -20°C for a long time. The traditi...

AI Technical Summary

Problems solved by technology

The traditional storage technology of mesenchymal stem cell exosomes adopts the method of storing and taking out multiple test tubes at the same time. In the process of taking a certain test tube, all the test tubes need to be exposed to room temperature. This method will seriously affect other The temperature during storage of test tubes containing exosomes of mesenchymal stem cells is constant, which reduces the storage effect of mesenchymal stem cells. In view of this situation, the present invention aims to design a kind of exosomes for mesenchymal stem cells that continuously maintains the storage temperature of each test tube. secretory storage technology

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