Tumor cell mass separation and filtration device and use method thereof

Abstract

The invention discloses a tumor cell mass separation and filtration device which comprises a bottom plate, two support plates are symmetrically and fixedly connected to the upper side of the bottom plate, and fixed boxes are fixedly connected to the upper ends of the support plates. The invention further provides a use method of the tumor cell mass separation and filtration device. The method comprises the following steps that S1, a double-head motor is started, and drives an eccentric wheel to rotate, the inertia of the eccentric wheel can make a transverse plate to move up and down, and thetransverse plate drives two first sliding rods to slide back and forth, and then a connecting block with a sieve mechanism to move up and down. The device is provided with a vibration mechanism and the sieve mechanism so that a first filter screen can jitter up and down and left and right, manual stirring can be omitted, the burden of operators is reduced, and cell damage is reduced; meanwhile, flowing is more smoothly, the device is provided with the two filter screens arranged vertically, large cell masses are removed for the first time, after separation, the cell masses are more uniform, the second filter screen on the lower layer, damaged single cells can be removed, and the separation quality is guaranteed.

Description

technical field [0001] The invention relates to the technical field of cell sorting and filtering, in particular to a sorting and filtering device for tumor cell clusters and an application method thereof. Background technique [0002] my country is a country with a high incidence of cancer in the world. The morbidity and mortality of cancer have been rising, and it has become one of the main causes of death among Chinese residents. The most common clinical malignant tumors are mainly solid malignant tumors. There are many reasons for inducing solid malignant tumors. At present, the pathogenesis of most solid malignant tumors is still unclear. Traditional cancer treatment mainly relies on surgery, chemotherapy, and radiotherapy. Chemotherapy, as the most commonly used systemic treatment, is widely used in various stages of cancer treatment. Plays a pivotal role in cancer treatment. However, due to the large individual differences in chemotherapy and the limited curative eff...

AI Technical Summary

Problems solved by technology

[0003] However, there is currently no special sorting and filtering device for tumor cell clusters on the market. Due to the uneven size of tumor cell clusters, cell clusters are easy to block the mesh of the filter. In order to make the flow more comfortable, the operator needs to stir continuously , which may cause cell damage and affect the quality of sorting. At the same time, the existing sorting mechanism cannot perform filtration. Generally, it needs to be repeated twice, which increases the workload of the operator.

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