Establishment method of peanut bacterial wilt resistance identification nursery

Abstract

The invention discloses a method for establishing a peanut bacterial wilt resistance identification nursery, which firstly separates, extracts and cultivates the bacterial wilt bacterial liquid from the peanut bacterial wilt disease strain, and uses a concentration of 3 to 5×10 7 Soak the susceptible peanut seeds in the R. solanacearum solution of cfu / ml, the soaking amount is 10 peanut seeds / 5ml, plant after soaking the seeds for 30 minutes; spray the R. solanacearum solution on the peanut leaves after the peanuts emerge, spray once every 10 days, and Wounded leaves were inoculated again with R. solanacearum; the peanut plants that died due to R. solanacearum were pulled out and crushed and sprinkled in the field, and the susceptible peanut seeds infected with R. After emergence, continue to inoculate with injured leaves, dead seedlings, and replant until harvesting, and the diseased plants are crushed and then returned to the field; two seasons are planted in spring and autumn every year, dead seedlings are replanted 2 to 4 times, and the above steps are repeated for 2 to 3 years to form peanut bacterial wilt Resistance identification nursery. It solves the problems of long establishment period, time-consuming and laborious establishment of peanut field disease resistance identification nursery.

Description

technical field [0001] The invention belongs to the field of bacterial wilt resistance identification and relates to a method for establishing a peanut bacterial wilt resistance identification nursery. Background technique [0002] Bacteria wilt is one of the important diseases in southern my country's peanut production areas. The incidence rate in the field is generally 10% to 30%, and it can reach more than 50% in severe cases. In addition to causing yield reduction, it also has an important impact on seed nutritional quality and aflatoxin pollution. Breeding bacterial wilt-resistant varieties is the most effective way to increase peanut yield. Disease-resistant breeding requires disease-resistant resources as breeding materials, and the existing resource resistance identification and screening technologies mainly rely on field disease identification. Field natural disease causes Due to uneven incidence and low incidence, the establishment cycle is long, time-consuming an...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a method for establishing a peanut bacterial wilt resistance identification nursery to solve the problems of long establishment period, time-consuming and laborious establishment of a peanut bacterial wilt resistance identification nursery