A colorimetric and electroluminescent dual-mode aptasensor and method for determining malathion

An aptamer sensor, malathion technology, applied in the measurement of color/spectral properties, chemiluminescence/bioluminescence, instruments, etc., can solve the problems of increased false alarm rate, increased uncertainty of detection results, etc., to reduce Error and false alarm rate, improved sensitivity and anti-interference ability, quick detection effect

Inactive Publication Date: 2021-11-16
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the single-sensing technology detection mode is generally used, which increases the uncertainty of the detection results and increases the false alarm rate.

Method used

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  • A colorimetric and electroluminescent dual-mode aptasensor and method for determining malathion
  • A colorimetric and electroluminescent dual-mode aptasensor and method for determining malathion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 CuFe 2 o 4 Preparation of MNCs:

[0040] (1) Weigh 0.3197 g CuCl 2 2H 2 O and 1.3500 g FeCl 3 ·6H 2 O was dissolved in 30.0 mL of ethylene glycol, and stirred vigorously for 10 min; then 2.700 g of NaAc and 0.75 g of polyethylene glycol 4000 were added, and the mixture was stirred vigorously for 30 min, and then sealed in an autoclave;

[0041] (2) The autoclave was heated to 200 °C for 8 h, then cooled to room temperature, washed with ultrapure water and redispersed.

Embodiment 2

[0042] Example 2 Apt / ALP-Au NPs@CuFe 2 o 4 Preparation of MNCs:

[0043] (1) Take 1.0 mL, 10 mg / mL CuFe 2 o 4 MNCs were mixed with 9.0 mL, 0.25 mmol / L Au NPs, stirred overnight and washed by centrifugation to obtain Au NPs@CuFe 2 o 4 MNCs composite materials;

[0044] (2) Add 300.0 µL, 100 µg / mL alkaline phosphatase ALP, 200.0 µL 1 µmol / L malathion aptamer Apt to 125.0 µL, 16.3 mg / mL Au NPs@CuFe 2 o 4 MNCs solution, incubated overnight, washed by centrifugation and redispersed into 1.0 mL Tris-HCl for storage.

Embodiment 3

[0045] Example 3 Preparation of three-dimensional gold gel / luminol nanocomposite modified glassy carbon electrode:

[0046] (1) Under stirring, add 1.2 mL of 0.1 mol / L sodium borohydride to 40.0 mL of 0.25 mmol / L sodium citrate dihydrate and 0.25 mmol / L HAuCl 4 Au NPs were obtained by aging for 6 h in the mixed solution;

[0047] (2) Then 1.5 mL of 0.1mol / L dopamine hydrochloride was added to the Au NPs solution and shaken for 30 min, then left to settle for 72 h and centrifuged to obtain a three-dimensional gold gel;

[0048] (3) Pipette 5.5 mL of the above three-dimensional gold gel solution and 0.5 mL, 60 mmol / L luminol, mix and shake overnight, centrifuge and wash to obtain the three-dimensional gold gel / luminol nanocomposite, and re- dispersion;

[0049] (4) Pipette 5.0 μL of the three-dimensional gold gel / luminol nanocomposite dispersion, drop-coat it on the surface of the treated glassy carbon electrode, and let it dry for later use.

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Abstract

The present invention relates to the technical field of pesticide residue analysis and determination, in particular to a colorimetric and electrochemiluminescence dual-mode aptamer sensor and a method for determining malathion by using the same, including the steps of preparing the dual-mode sensor and using the sensor to determine The operation method of malathion, etc.; this dual-mode sensor, using a 96-well plate, is easy to operate, fast for visual inspection, and can perform qualitative and semi-quantitative detection; The visible spectrometer can detect the content of malathion, and verify the results with each other, with high accuracy; this method effectively reduces the error and false alarm rate caused by a single mode, and has little interference from coexisting substances, good selectivity and high sensitivity.

Description

technical field [0001] The invention relates to the technical field of analysis and determination of pesticide residues, in particular to a dual-mode sensor based on colorimetry and electrochemiluminescence and a method for determining malathion using it, including the preparation steps of the dual-mode sensor and the determination of malathion using the sensor. The operation method of parathion, etc.; this dual-mode sensor can be used for qualitative and semi-quantitative detection by visual colorimetry; the content of malathion can be detected with the help of MPI-B multi-parameter chemiluminescence analysis test system and ultraviolet-visible spectrometer, and The results are mutually confirmed; the error and false alarm rate produced by a single model are effectively reduced. Background technique [0002] Among the conventional analytical methods for pesticide residues, chromatography and its combined techniques still play an important role due to their high sensitivity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N27/30G01N27/48G01N21/76G01N21/31
CPCG01N21/3151G01N21/76G01N21/78G01N27/308G01N27/48G01N2021/3155
Inventor 周长利刘汉彪陈培培刘建辉夏方诠田栋
Owner UNIV OF JINAN
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