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Gene sequencing chip, equipment and preparation method

A gene sequencing and chip technology, applied in biochemical equipment and methods, microbial measurement/testing, biochemical instruments, etc., can solve problems such as improving and unfavorable gene sequencing accuracy

Pending Publication Date: 2019-09-13
BOE TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only a small amount of light emitted to the medium other than the side wall of the sequencing tank can be detected, which is not conducive to the further improvement of the accuracy of gene sequencing

Method used

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  • Gene sequencing chip, equipment and preparation method
  • Gene sequencing chip, equipment and preparation method
  • Gene sequencing chip, equipment and preparation method

Examples

Experimental program
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Embodiment 1

[0030] refer to Figure 1-3 , the present embodiment provides a gene sequencing chip, comprising: a substrate body 1, at least one sequencing slot 2 is arranged on the first side of the substrate body 1, and a sampling slot is arranged on the first side of the substrate body 1 and in an area surrounding the sequencing slot 2. The light structure 3 , the light-trapping structure 3 is used to take out the light in the substrate body 1 and directed to the light-trapping structure 3 from the first side of the substrate body 1 .

[0031] The substrate main body 1 may be formed of a single material, or may be formed of stacked or mixed layers of materials.

[0032] When performing gene sequencing, the gene 4 to be tested is located in the sequencing slot 2 , and the gene 4 to be tested is marked with a specific fluorescent group 5 . by image 3 At the current viewing angle, most of the light emitted by the excited fluorophore 5 is emitted upward through the notch of the sequencing...

Embodiment 2

[0049] This embodiment provides a gene sequencing device, including: a gene sequencing chip, which uses the gene sequencing chip provided in Example 1.

[0050] The gene sequencing equipment detects more accurately the light emitted from the side of the gene sequencing chip, thereby making the result of gene sequencing more accurate.

Embodiment 3

[0052] refer to Figure 4 and combine Figure 1-3, this embodiment provides a method for preparing a gene sequencing chip, the gene sequencing chip is the gene sequencing chip of embodiment 1, and the preparation method includes:

[0053] Step S1, providing a substrate body.

[0054] Step S2, forming sequencing slots on the first side of the substrate body, and forming light-taking structures on the first side of the substrate body.

[0055] The gene sequencing chip prepared according to this manufacturing method can utilize the light emitted by the fluorescent group 5 on the gene 4 to be tested subsequently placed in it to a greater extent, thereby improving the accuracy of gene sequencing.

[0056] For the specific description of the sequencing slot 2 and the light-taking structure 3 formed in step S2, reference may be made to the corresponding content in the foregoing embodiments, and details will not be repeated here.

[0057] Optionally, in a specific example, the deta...

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Abstract

The invention discloses a gene sequencing chip, equipment and a preparation method. The gene sequencing chip comprises a substrate main body, wherein at least one sequencing slot is formed in the first side of the substrate main body; and a light extraction structure is arranged on the first side of the substrate main body and in the region enclosing the sequencing slot, and is used for extractingthe light which is positioned in the substrate main body and hits the light extraction structure from the first side of the substrate main body. The gene sequencing chip can further improve the accuracy of gene detection.

Description

technical field [0001] The invention relates to the field of gene sequencing, in particular to a gene sequencing chip, a gene sequencing device, and a method for preparing the gene sequencing chip. Background technique [0002] In the existing gene sequencing chip, generally, a sequencing slot (or called a sequencing hole) is arranged on the main body of the substrate. A certain type of base (one of adenine, thymine, cytosine, and guanine) labeled with a fluorescent group is excited to emit light of a specific color under the condition of light of a specific wavelength. By detecting the light emitted by the excited fluorophore, the order of the bases in the gene can be inferred. Part of the light emitted by the fluorophore in the sequencing slot is directly emitted from the opening of the sequencing slot, and part of it is emitted into the medium other than the side wall of the sequencing slot, and finally reflected or totally reflected on the top surface of the medium. On...

Claims

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Application Information

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IPC IPC(8): C12M1/34
CPCC12Q1/6869
Inventor 张笑谷新
Owner BOE TECH GRP CO LTD
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