Application of PRDM1 as marker for preparing product for diagnosing active tuberculosis

A PRDM1, tuberculosis technology, applied in the fields of disease diagnosis, biochemical equipment and methods, microbial determination/examination, etc., can solve problems such as no research reports yet

Inactive Publication Date: 2019-10-01
中国人民解放军总医院第八医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies on the relationship between PRDM1 and tuberculosis, and there is no research report on the use of PRDM1 gene expression in the diagnosis of active tuberculosis.

Method used

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  • Application of PRDM1 as marker for preparing product for diagnosing active tuberculosis
  • Application of PRDM1 as marker for preparing product for diagnosing active tuberculosis
  • Application of PRDM1 as marker for preparing product for diagnosing active tuberculosis

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0086] Example 1. Detection of PRDM1 gene expression level in PBMCs of patients with active tuberculosis and patients with latent tuberculosis infection and its correlation analysis

[0087] 1. Experimental materials and methods

[0088] 1. Obtaining peripheral blood samples

[0089] Peripheral blood samples of subjects: peripheral blood samples from 140 patients and 31 healthy subjects. The 140 patients consisted of 109 patients with active tuberculosis and 31 patients with latent tuberculosis infection. Among them, the specific composition of the 109 patients with active tuberculosis was as follows: 38 cases of clinically confirmed positive pulmonary tuberculosis patients, 53 cases of clinically confirmed Bacterial negative pulmonary tuberculosis patients and 18 clinically diagnosed extrapulmonary tuberculosis patients.

[0090] The method of obtaining peripheral blood samples is as follows: 2-3 mL of peripheral blood were drawn from 140 patients and 31 healthy subjects (a...

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PUM

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Abstract

The invention discloses application of PRDM1 as a marker for preparing a product for diagnosing active tuberculosis. Fluorescence quantitative PCR is adopted for detecting expression quantity of a PRDM1 gene in a tuberculosis patient PBMCs. A result shows that the relative expression quantity of PRDM1 in smear positive tuberculosis patients, smear negative tuberculosis patients and extrapulmonarytubereulosis patients PBMCs is remarkably lowered that that of latent tuberculosis infected persons and healthy control. A subject working characteristic curve analysis result shows that PRDM1 can distinguish active tuberculosis and latent tuberculosis infection, and becomes the diagnosis target for diagnosing the active tuberculosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of PRDM1 as a marker in the preparation of products for diagnosing active tuberculosis. Background technique [0002] Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (M.tb) infection, which seriously threatens human health. After adults are infected with Mycobacterium tuberculosis, some of them will live in a carrier state and will not develop tuberculosis, which is called latent tuberculosis infection (LTBI); about 5-10% of latent infections will continue to develop active tuberculosis. The results of epidemiological surveys in various regions in China show that the infection rate of Mycobacterium tuberculosis based on the gamma-interferon release assay (IGRA) is about 20-30%, that is, about 300-400 million people in the country are mycobacterium tuberculosis. Bacillus infection, a large number of asymptomatic tuberculosis late...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68G16B30/00
CPCC12Q1/6883G01N33/6893G16B30/00C12Q2600/158G01N2333/47G01N2800/26
Inventor 刘艳华程小星王若杨秉芬蒋静苏瑾文翟斐曹志红安红娟
Owner 中国人民解放军总医院第八医学中心
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