Bifidobacterium adolescentis ccfm1061, its fermented food and preparation method of bacterial agent

A bifidobacterium, fermented food technology, applied in bifidobacteria, bacteria used in food preparation, biochemical equipment and methods, etc., can solve liver function damage and edema, increased toxic and side effects of drugs, adverse reactions in the digestive tract, etc. problems, to achieve the effect of alleviating the toxicity of PFOA, reducing absorption and improving antioxidant capacity

Active Publication Date: 2020-12-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have a certain therapeutic effect, but as the disease worsens, the dosage increases, drug interactions, and drug side effects also increase significantly, leading to adverse reactions in the digestive tract and showing certain liver and kidney toxicity, such as Long-term use of metformin can cause discomfort in the gastrointestinal tract of some patients and may affect the absorption of vitamin B12. Rosiglitazone can cause liver damage and edema, etc.

Method used

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  • Bifidobacterium adolescentis ccfm1061, its fermented food and preparation method of bacterial agent
  • Bifidobacterium adolescentis ccfm1061, its fermented food and preparation method of bacterial agent
  • Bifidobacterium adolescentis ccfm1061, its fermented food and preparation method of bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Characteristics of Bifidobacterium adolescentis CCFM1061:

[0046] (1) Bacterial characteristics: Gram-positive bacteria that do not form spores and do not move;

[0047] (2) Colony characteristics: After 36 hours of anaerobic culture, obvious colonies are formed, with a diameter between 0.5-1mm, a round front shape, a protruding side shape, neat edges, milky white, translucent, moist and smooth surface, and no pigmentation , see attached figure 1 ;

[0048] (3) Growth characteristics: under the condition of constant temperature and anaerobic at 37° C., cultivate in the modified mMRS medium for about 20 hours to reach the end of logarithmic phase.

[0049] (4) Significantly increase the abundance of Akkermansia in the intestinal tract of NAFLD mice, and reduce the occurrence of diseases such as obesity, diabetic fatty liver and enteritis;

[0050] (5) Significantly improve lipid metabolism disorder in NAFLD mice;

[0051] (6) Significantly improved insulin resistanc...

Embodiment 2

[0093] Example 2: Bifidobacterium adolescentis CCFM1061 has no toxic side effects on C57BL / 6J mice

[0094] The Bifidobacterium adolescentis CCFM1061 bacteria were resuspended in 3% sucrose solution to make a concentration of 3.0×10 9CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 16-20 g were taken. After one week of adaptation to the environment, 0.2 mL of Bifidobacterium adolescentis CCFM1061 was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0095] The results of these tests are listed in Table 1. These results indicate that daily feeding of 0.2 mL of 3.0×10 9 CFU / mL of Bifidobacterium adolescentis CCFM1061 had no significant effect on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0096] Table 1 Changes in body weight and death of mice

[0097]

[0098] Note: -: no death of mice

Embodiment 3

[0099] Example 3: Regulatory effect of Bifidobacterium adolescentis CCFM1061 on intestinal flora of NAFLD mice

[0100] Take 48 healthy male C57BL / 6J mice weighing 16-20g, adapt to the environment for 1 week, and randomly divide them into 6 groups: blank control group (NC), model control group (M), rosiglitazone control group (RC) , simvastatin control group (SC), Bifidobacterium adolescentis CCFM1061 intervention group (CCFM1061), Lactobacillus rhamnosus L10 intervention group (LC), each group contains 8 mice. The experimental animal groups and treatment methods are shown in Table 2:

[0101] Table 2 Grouping of experimental animals

[0102]

[0103]

[0104] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the metagenome in the feces was extracted, and the structure of the intestinal flora was analyzed using a next-generation sequencer. At the end of the experiment, the mice were fasted for 12 hours, anesthetized by intrap...

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Abstract

The invention discloses Bifidobacterium adolescentis CCFM1061, its fermented food and a preparation method of bacterial preparations. Bifidobacterium adolescentis CCFM1061 can significantly improve liver damage in patients with non-alcoholic fatty liver disease, reduce serum ALT levels, and reduce hepatic steatosis and hepatic ballooning. lesions and hepatic lobular inflammation; it can significantly alleviate the increase in serum total cholesterol, blood sugar, liver triglyceride and low-density protein cholesterol caused by high-fat diet in patients with nonalcoholic fatty liver disease; at the same time, it can significantly improve liver It can significantly increase the expression of Nrf2 gene in fatty liver cells; improve the fasting blood sugar in type 2 diabetic mice; in addition, Bifidobacterium adolescent CCFM1061 has a strong adsorption capacity for PFOA, which reduces the absorption of PFOA in the body and has the effect of relieving PFOA toxicity capacity.

Description

technical field [0001] The invention belongs to the technical field, and in particular relates to bifidobacterium adolescent CCFM1061, its fermented food and a preparation method of bacterial agent. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is fatty degeneration caused by excessive accumulation of fat in liver cells. It is a symptom of liver metabolic disorders. Without intervention, it can gradually evolve into non-alcoholic fat Hepatitis (NASH) further deteriorates into liver fibrosis, liver cirrhosis, and liver cancer with extremely high mortality rates. In recent years, with the improvement of living standards, more and more high-fat and high-energy diets are put on the table, but people's exercise has not increased accordingly, which makes metabolic syndrome represented by non-alcoholic fatty liver disease The incidence of symptoms is increasing year by year. Due to its imperfect pathological mechanism, there are no drugs specifically tar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/133A23C20/02A23L33/00C12R1/01
CPCC12N1/20A23C9/133A23C20/025A23L33/00C12R2001/01C12N1/205A23V2400/513
Inventor 陈卫王刚焦婷赵建新张灏
Owner JIANGNAN UNIV
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