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Application and kit of detection reagents for detecting colorectal cancer-related gene methylation

A technology for colorectal cancer and detection reagents, applied in biochemical equipment and methods, microbe determination/testing, DNA/RNA fragments, etc., can solve the problem of low detection sensitivity

Active Publication Date: 2020-05-26
WUHAN AIMISEN LIFE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The problems and shortcomings of this method are: the patent uses 46 cases of colorectal cancer, 46 cases of colorectal adenoma and 46 cases of normal stool samples for SDC2 methylation detection, and the results show that SDC2 is more sensitive to colorectal cancer than cancer. low, only 87%

Method used

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  • Application and kit of detection reagents for detecting colorectal cancer-related gene methylation
  • Application and kit of detection reagents for detecting colorectal cancer-related gene methylation
  • Application and kit of detection reagents for detecting colorectal cancer-related gene methylation

Examples

Experimental program
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Effect test

Embodiment 1

[0082] This embodiment provides a kit for auxiliary diagnosis of colorectal cancer or colorectal adenoma, which includes: detection reagents for detecting methylation of colorectal cancer-related genes, wherein the colorectal cancer-related gene is the SDC2 gene, The detection reagents include a first reagent for detecting methylation of a first target region on the SDC2 gene;

[0083] The first target region is selected from any one of the following regions of the SDC2 gene: region a, region b and region c;

[0084] Among them, refer to figure 1, region a is selected from the full-length region of chr8:96493351-96493635 (hereinafter referred to as SDC2DMR1, it should be noted that, in other embodiments, region a can be selected from a partial region of chr8:96493351-96493635, whether it is the detection The full length of the chr8:96493351-96493635 region or a part of it, all belong to the protection scope of the present invention);

[0085] Region b is selected from the fu...

Embodiment 2

[0095] The kit for auxiliary diagnosis of colorectal cancer or colorectal adenoma provided in this example is basically the same as that in Example 1, the difference is that in this example:

[0096] Area a is selected from the partial area of ​​SDC2 DMR 1, specifically, refer to figure 1 , region a is selected from chr8:96493393-96493581 (named SDC2 DMR 1-a) or chr8:96493456-96493629 (named SDC2 DMR 1-a1);

[0097] Area b is selected from the partial area of ​​SDC2 DMR 2, specifically, refer to figure 1 , region b is selected from chr8:96493701-96493858 (named SDC2 DMR 2-b) or 96493770-96493900 (named SDC2 DMR 2-b1);

[0098] Area c is selected from the partial area of ​​SDC2 DMR 3, specifically, refer to figure 1 , region c is selected from chr8:96494006-96494166 (named SDC2 DMR 3-c) or chr8:96494140-96494327 (named SDC2 DMR 3-c1).

[0099] Furthermore, the kit of this embodiment also includes primer pairs and fluorescent probes for detecting the above target regions, and...

Embodiment 3

[0105] This embodiment provides a kit for the auxiliary diagnosis of colorectal cancer or colorectal adenoma, which includes: a detection reagent for detecting the methylation of colorectal cancer-related genes, wherein the colorectal cancer-related gene is the TFPI2 gene, The detection reagents include a second reagent for detecting methylation of a second target region on the TFPI2 gene;

[0106] Wherein, the second target region is selected from any one of the following regions of the TFPI2 gene: region d and region e;

[0107] Among them, refer to figure 2 , region d is selected from the full-length region of chr7:93890026-93890319 (hereinafter referred to as TFPI2 DMR1, it should be noted that, in other embodiments, region d can be selected from a partial region of TFPI2 DMR1, whether it is to detect chr7: The entire length of the 93890026-93890319 area or a part of it, all belong to the protection scope of the present invention);

[0108] Region e is selected from the...

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Abstract

The invention discloses an application and a kit of detection reagents for detecting colorectal cancer-related gene methylation, and relates to the technical field of gene detection. The kit disclosed in the present invention includes detection reagents for detecting the methylation of colorectal cancer-related genes, by performing methylation detection on specific regions in the SDC2 gene and TFPI2 gene, and comparing the methylation detection results of these regions As the basis for auxiliary diagnosis of colorectal cancer, it has higher sensitivity and specificity, and provides a more accurate and reliable reference for early screening and diagnosis of colorectal cancer or colorectal adenoma.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to the application and kit of detection reagents for detection of colorectal cancer-related gene methylation. Background technique [0002] Colorectal cancer is the third most common malignancy in the world. In recent years, the incidence of colorectal cancer in China has been increasing year by year. It is estimated that there are about 400,000 new cases each year, ranking second among malignant tumors of the digestive system in my country. Colorectal cancer usually develops slowly from precancerous polyps through a series of genetic and epigenetic alterations as well as histological and morphological changes, and most colorectal tumors grow slowly, remain silent, or are asymptomatic until they reach the A certain size or stage of development. Therefore, colorectal cancer is an ideal target for intervention through screening. The 5-year survival rate of patients with earl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154C12Q1/6806
Inventor 张良禄李婷婷董兰兰吴志诚姚希辉陈玉珠
Owner WUHAN AIMISEN LIFE TECH CO LTD
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