Cell sample liquid feed bag, cell sample liquid feed method, and cell sample liquid feed device
A liquid supply and supply bag technology, applied in measuring devices, chemical instruments and methods, biochemical cleaning devices, etc., can solve problems such as disturbance and dead volume
Inactive Publication Date: 2019-10-18
SONY CORP
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- Abstract
- Description
- Claims
- Application Information
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Problems solved by technology
[0005] Here, a general cell sample liquid supply bag has a quadrangular shape, the liquid inlet / outlet has a large pipe diameter, and there is a problem of air mixture when the cell sample liquid inside the bag is sucked out, and there is also a problem of air mixture due to remaining in the corners of the bag. The problem of liquids creating dead volume
Additionally, the air mixture is desirable to prevent the phenomenon of feeding the cell sample liquid to the particle measurement device, because the air mixture becomes severely disturbed when the signal of the cell particles is found
Also, since the sample is discarded, dead volume must be minimized
Method used
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[0059] Preferred embodiments for carrying out the present technology will be described below with reference to the accompanying drawings. It should be noted that the embodiments described below illustrate representative embodiments of the present technology and the scope of the present technology should not be construed as being limited by these embodiments. It should be noted that description will be provided in the following order.
[0060] 1. Cell sample liquid supply bag 1
[0061] 2. Cell sample liquid supply method
[0062] (1) Injection process
[0063] (2) Other processes
[0064] 3. Cell sample liquid supply device 10
[0065] 4. Microparticle measuring device 100
[0066] (1) Flow path P
[0067] (1-1) Particle measurement chip M
[0068] (2) Fluid control unit 101
[0069] (3) Light emitting unit 102
[0070] (4) Photodetection unit 103
[0071] (5) Analysis unit 104
[0072] (6) Classification unit 105
[0073] (7) Storage unit 106
[0074] (8) Display...
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The objective of the present technology is to provide a technique capable of performing stable liquid feed. To this end, the present technology provides a cell sample liquid feed bag and the like including an outflow port through which a cell sample liquid flows out, and a storage portion capable of storing cells, wherein the cell sample liquid feed bag is provided at least with a bottom portion having a gradient in at least part thereof, and a first inner tube extending from the outflow port toward the storage portion as far as a position not in contact with the storage portion. The cell sample liquid is fed from the storage portion side of the first inner tube toward the outflow port side thereof.
Description
technical field [0001] The technology relates to a cell sample liquid supply bag, a cell sample liquid supply method and a cell sample liquid supply device. Background technique [0002] In recent years, regenerative medicine / cell therapy is being actively studied and demand for flow cytometry as a technique for rapidly evaluating cells is increasing. Flow cytometry is an analysis technique in which particles to be analyzed are poured into a fluid in an aligned state, and the particles are analyzed and classified by irradiating the particles with laser light or the like and detecting fluorescence and scattered light emitted from each particle, and flow cytometry Cytometers are used as tools for analyzing cells in research into regenerative medicine / cell therapy. As a device of the above-mentioned flow cytometer, for example, microparticle measuring devices disclosed in Patent Documents 1 to 4 are known. [0003] Conventionally, it is known that in the case of supplying the...
Claims
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IPC IPC(8): G01N1/00C12M1/00G01N15/00G01N15/14
CPCC12M1/00G01N1/00G01N15/00G01N15/14B01L3/505B01L3/502776B01L3/502784B01L2200/026B01L2200/0689B01L2200/0673B01L2200/141B01L2300/0858B01L2300/123B01L2200/0652C12M23/14C12M23/20C12M23/34C12M29/00G01N15/1404G01N15/10
Inventor 田代真司桥本学治高桥和也胜本洋一坂本直久
Owner SONY CORP