Biological active scaffold and preparation method thereof

A bioactive and buffer technology, applied in the field of micro/nano bioactive scaffolds with supramolecular recognition functions, can solve the problems of difficulty in ensuring activity and lack of biological carriers, and achieve the effects of promoting repair, preventing cerebrospinal fluid leakage, and promoting migration behavior

Pending Publication Date: 2019-10-22
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a micro/nano bioactive scaffold with supramolecular recognition function, which

Method used

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  • Biological active scaffold and preparation method thereof
  • Biological active scaffold and preparation method thereof
  • Biological active scaffold and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A bioactive scaffold includes the following components: silk fibroin and Col-I, and the mass ratio of the silk fibroin to Col-I is 15:1.

[0037] The dry silk fibroin was dissolved in 7% w / t hexafluoroisopropanol to obtain a silk fibroin solution, and the silk fibroin solution was electrospun at an injection speed of 0.8 ml / min, a voltage of 12 kV, and a jet collection distance of 10 cm Silk treatment, after electrospinning, soak in anhydrous methanol, wash with deionized water, and freeze-dry to obtain SF electrospun membrane. Add the SF electrospun membrane to the tris-HCl buffer solution of dopamine (concentration 2 mg / ml, pH=8.5) and soak for 12 h.

[0038] Dissolve Col-I in 0.006 mmol / L acetic acid at a concentration of 3 mg / L, mix it with 10×PBS solution at a ratio of 1:6, and adjust the pH to 7.0.

[0039] Drop Col-I onto the SF electrospun membrane, place it at 37°C for 30 min, and wash it with deionized water to obtain the Col-I modified SF electrospun membran...

Embodiment 2

[0042] A bioactive scaffold includes the following components: silk fibroin and Col-I, and the mass ratio of the silk fibroin to Col-I is 15:1.

[0043]The silk fibroin solution was obtained by dissolving the dry silk in 6% w / t hexafluoroisopropanol, and the silk fibroin solution was subjected to electrospinning treatment at an injection speed of 0.6ml / min, a voltage of 15 kV, and a spray collection distance of 15 cm. After spinning, soak in anhydrous methanol, wash with deionized water, and freeze-dry to obtain SF electrospun membrane. The SF electrospun membrane was soaked in tris-HCl buffer solution of dopamine (concentration 3 mg / ml, pH=8.6) for 12 h.

[0044] Dissolve Col-I in 0.009 mmol / L acetic acid at a concentration of 5 mg / L, mix it with 10×PBS solution at a ratio of 1:6, and adjust the pH to 7.0.

[0045] Drop Col-I onto the SF electrospun membrane, place it at 37°C for 30 min, and wash it with deionized water to obtain the Col-I modified SF electrospun membrane, w...

Embodiment 3

[0047] A bioactive scaffold includes the following components: silk fibroin and Col-I, and the mass ratio of the silk fibroin to Col-I is 15:1.

[0048] The silk fibroin solution was obtained by dissolving the dry silk in 9% w / t hexafluoroisopropanol. The silk fibroin solution was subjected to electrospinning at a sampling rate of 1.0ml / min, a voltage of 16 kV, and a jet collection distance of 8 cm. After spinning, soak in anhydrous methanol, wash with deionized water, and freeze-dry to obtain SF electrospun membrane. The SF electrospun membrane was soaked in tris-HCl buffer solution of dopamine (concentration 1 mg / ml, pH=8.2) for 12 h.

[0049] Dissolve Col-I in 0.003mmol / L acetic acid at a concentration of 1 mg / L, mix it with 10×PBS solution at a ratio of 1:6, and adjust the pH to 7.0.

[0050] Drop Col-I onto the SF electrospun membrane, place it at 37°C for 30 min, and wash it with deionized water to obtain the Col-I modified SF electrospun membrane, wherein the mass rati...

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Abstract

The invention discloses a biological active scaffold and a preparation method thereof, which belong to the field of biological tissue engineering. The biological active scaffold includes silk fibroinand type I collagen. The method for preparing the biological active scaffold comprises the steps of: dissolving a SF electrospun membrane in a dopamine Tris-HCl buffer solution, and soaking the materials for 12 h; dissolving Col-I in acetic acid to obtain a Col-I acetic acid solution, and then adding a 10*PBS solution to adjust the pH of the solution to neutrality; and dropping the Col-I onto theSF electrospun membrane to obtain the biological active scaffold. The biological active enables the Decorin load of the SF electrospun fiber membrane. The formation of epidural scars can be inhibited.

Description

technical field [0001] The invention belongs to the field of biological tissue engineering, and in particular relates to a micro / nano bioactive scaffold with supramolecular recognition function. Background technique [0002] Dural defect caused by trauma, tumor and iatrogenic injury is a common problem in spinal and neurosurgery. Dural defects lead to cerebrospinal fluid leakage, often leading to complications such as infection, fistula formation, peridural adhesions, or pseudomeningocele, which seriously reduce the quality of life of patients and bring a huge burden to society. Autologous tissue is commonly used as traditional dural repair materials, but it is difficult and limited in quantity. There is a risk of transmission of Creutzfeldt-Jakob disease or other infectious diseases with allogeneic dura mater restoration materials. At present, the main component of heterogeneous dura mater replacement materials in clinical application is animal-derived collagen. Its main ...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/22A61L27/50A61L27/54A61L27/58A61K9/70A61K47/42A61P25/00A61P17/02D01D5/00D04H1/728D04H1/42
CPCA61K9/7007A61K47/42A61L27/227A61L27/24A61L27/50A61L27/54A61L27/58A61L2400/12A61L2430/32A61P17/02A61P25/00D01D5/003D01D5/0061D01D5/0092D04H1/42D04H1/728C08L89/00
Inventor 陈亮顾勇许运钱明崔文国
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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