Unlock instant, AI-driven research and patent intelligence for your innovation.

Anti-human st2 antibody and use thereof

An antibody and sequence technology, applied in the direction of antibodies, anti-animal/human immunoglobulins, applications, etc., can solve the problems of no transmembrane domain and unclear functions

Active Publication Date: 2020-09-18
BEIJING WISDOMAB BIOTECHNOLOGY CO LTD
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ST2 has four splice variants, one is the transmembrane protein ST2L, which is mainly expressed in mast cells, Th2 lymphocytes and eosinophils, and forms IL-33 receptor complex with IL-1RAcP (also known as IL-1R3) one is a short secreted splicing variant sST2, the C-terminus has 9 more amino acid residues than the extracellular region of ST2L, it is mainly expressed in embryonic tissues, breast tumors and fibroblasts, and is an antagonistic pseudotype of IL-33 receptor, inhibits IL-33-mediated signaling; a third variant of unknown function expressed in the stomach, small intestine, and colon, called ST2V, differs from sST2 only in the third extracellular Ig domain; Four ST2LVs are similar to ST2L but lack the transmembrane domain

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-human st2 antibody and use thereof
  • Anti-human st2 antibody and use thereof
  • Anti-human st2 antibody and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0157] Example 1: Construction of phage display antibody library

[0158] For specific experimental procedures, please refer to Example 1 of Chinese Patent Application No. 201610609651.X and Example 1 of Chinese Patent Application No. 201510097117.0. The entire contents of the above two patent applications are incorporated herein by reference.

Embodiment 2

[0159] Embodiment 2: Preparation of recombinant protein

[0160] A variety of different recombinant proteins are used in the preparation of anti-ST2 monoclonal antibodies, including human ST2 extracellular region (hST2, SEQ ID NO:1), mouse ST2 extracellular region (mST2, SEQ ID NO:2) , macaque ST2 extracellular region (mmST2, SEQ ID NO:3) and human IL-33 extracellular region (hIL-33, SEQ ID NO:4). These proteins have a large number of post-translational modifications (such as glycosylation or disulfide bonds, etc.), so the use of mammalian cell expression systems will be more conducive to maintaining the structure and function of recombinant proteins. In addition, a His tag (His, SEQ ID NO:5) or the Fc fragment of human IgG1 (hFc, SEQ ID NO:6) or the Fc fragment of mouse IgG2a (mFc, SEQ ID NO:6) was added to the C-terminus of these recombinant proteins. NO:7), which will be more conducive to the purification of recombinant proteins and the identification of monoclonal antibod...

Embodiment 3

[0163] Example 3: Screening of anti-human ST2 monoclonal antibodies using phage display antibody library technology

[0164] 3.1 Screening of anti-human ST2 monoclonal antibody

[0165] Using the recombinant human ST2-his prepared in Example 2 as an antigen, a solid-phase screening strategy (experimental protocol refers to phage display: general experimental guidelines / (US) Clarkson (Clackson, T.), (US) Loman (Lowman, Edited by H.B.; Translated by Ma Lan et al. Chemical Industry Press, 2008.5) Screened the phage library displaying the human single-chain antibody library prepared in Example 1, and finally obtained a fully human single-chain antibody S1A4 that specifically binds to human ST2.

[0166] 3.2 Preparation of fully human antibody

[0167] Using conventional molecular biology methods, the nucleic acid molecules encoding the heavy chain variable region of S1A4 (S1A4 VH, SEQ ID NO:18) and the light chain variable region (S1A4 VK, SEQ ID NO:19) were respectively cloned i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed in the present application are an antibody or an antigen-binding portion thereof binding to human ST2, a polynucleotide encoding the antibody or the antigen-binding portion thereof, a vector containing the polynucleotide, a host cell containing the polynucleotide or the vector, a method for preparing and purifying the antibody, and an application of the antibody or the antigen binding portion thereof.

Description

technical field [0001] The present application generally relates to the field of genetic engineering and antibody medicine; specifically, it relates to the field of anti-human ST2 antibody and its application. The present application develops a new anti-human ST2 antibody, and provides the use of the antibody in the prevention or treatment of ST2-mediated diseases. Background technique [0002] Growth Stimulation Expressed Gene 2 (ST2), also known as IL1RL1, T1, DER4 or Fit-1, is a member of the IL-1 receptor family and belongs to the Toll-like receptor (TLR) / IL- The 1R (TIR) ​​superfamily is a selective marker for Th2 lymphocytes and an important effector molecule for Th2 responses. The ST2 gene was first discovered by Tominaga et al. in 1989 when they were studying the BALB / c-3T3 cell line, and it was considered an orphan receptor for a long time until Schmitz et al. discovered that IL-33 can specifically activate ST2, and confirmed that it is a functional ligand of ST2....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28C12N15/13A61K39/395A61P37/02A61P29/00A61P19/02A61P11/00A61P13/12A61P17/00A61P11/06A61P11/02A61P1/00A61P19/08A61P25/02
CPCA61K2039/505A61P1/00A61P11/00A61P11/02A61P11/06A61P13/12A61P17/00A61P19/02A61P19/08A61P25/02A61P29/00A61P37/02C07K16/2866C07K2317/33C07K2317/56C07K2317/565C07K2317/76C07K2317/92
Inventor 刘志刚刘玉兰郝小勃郭晶晶
Owner BEIJING WISDOMAB BIOTECHNOLOGY CO LTD