Reagent for multiple detection of salmon trout virus and application thereof

A technology for multiple detection and salmon trout, applied in the field of salmon trout virus detection, can solve the problems of time-consuming and laborious, multi-target detection is prone to false positive results, and time-consuming, so as to shorten the detection time and improve the detection efficiency and quality. Effect

Active Publication Date: 2019-10-29
SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the virus isolation method has accurate detection results, but it takes a long time and requires high experimental conditions; ordinary PCR and real-time fluorescent PCR detection methods have greatly improved the sensitivity and accuracy of detection, but currently only work well for a single target. False-positive results a

Method used

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  • Reagent for multiple detection of salmon trout virus and application thereof
  • Reagent for multiple detection of salmon trout virus and application thereof
  • Reagent for multiple detection of salmon trout virus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0071] 1. Materials and methods

[0072] 1.1 Main test materials

[0073] The main test materials used in this example include, Qiagen One Step RT-PCR Kit and EZ1 Virus miniKit2.0 were purchased from Qiagen Company, and phycoerythrin (SAPE) was purchased from Invitrogen Company of the United States. Liquichip carboxybead was purchased from Luminex Company, and Luminex calibration bead mix and Luminex control beadkit were purchased from Qiagen Company.

[0074] 1.2 Main instruments

[0075] A small fully automatic nucleic acid extraction workstation (BioRobot EZ1 Workstation) was purchased from Qiagen, a high-speed desktop refrigerated centrifuge (Allegra 64R centrifuge) was purchased from Beckman, and a liquid chip detection workstation (Liquichip 200) was purchased from Qiagen.

[0076] 1.3 Test virus material

[0077] The test materials in this example include infectious hematopoietic necrosis virus (Infectious hematopoieticnecrosis virus, IHNV) nucleic acid samples, infe...

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Abstract

The invention discloses a reagent for multiple detection of salmonidae virus and an application thereof. The reagent provided by the invention comprises a specific primer group, wherein the specific primer group comprises at least one pair from a first primer pair to a sixth primer pair; the first primer pair to the sixth primer pair are sequentially primer pairs of: nucleic acid of specifically-amplified spring viraemia of carp virus, nucleic acid of infectious hematopoietic necrosis virus, nucleic acid of viral haemorrhagic septicaemia virus, nucleic acid of infectious salmon anaemia virus,nucleic acid of salmonid alphavirus, and nucleic acid of infectious pancreatic necrosis virus. The upstream and downstream primers of the first primer pair to the sixth primer pair are sequentially the sequences as shown in Seq ID No.1 to Seq ID No.12. The reagent can carry out multiple detection on six salmonidae viruses, is simple and convenient to use, shortens the detection time of the six salmonidae viruses, improves the detection efficiency and quality, and has great significance for rapid detection and prevention and control of the six salmonidae viruses.

Description

technical field [0001] The application relates to the field of salmon trout virus detection, in particular to a reagent for multiple detection of salmon trout virus and its application. Background technique [0002] Salmon and trout are the general term for fish in the family Salmonidae, which includes all species of the subfamily Salmonidae and the subfamily Salmonidae. Salmon trout enjoys the reputation of "water ginseng" because of its tender meat, delicious taste and high nutritional value. It is a high-quality cultured species promoted by the Food and Agriculture Organization of the United Nations to countries all over the world. China began to introduce salmon and trout farming in the 1970s. At present, the salmon and trout farming area has reached more than 1,500 mu, and the annual production of salmon and trout is about 30,000 tons. It is one of the main cold-water fish farming species in China. [0003] In recent years, salmon and trout diseases have occurred frequ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6837C12N15/11
CPCC12Q1/6837C12Q1/701C12Q2600/16C12Q2531/113C12Q2537/143C12Q2563/107C12Q2565/501
Inventor 郑晓聪陈雨孙洁刘莹史秀杰温智清韦信贤贾鹏王津津刘荭秦智锋
Owner SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT
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