Reagent for multiple detection of salmon trout virus and its application

Abstract

The invention discloses a reagent for multiple detection of salmonidae virus and an application thereof. The reagent provided by the invention comprises a specific primer group, wherein the specific primer group comprises at least one pair from a first primer pair to a sixth primer pair; the first primer pair to the sixth primer pair are sequentially primer pairs of: nucleic acid of specifically-amplified spring viraemia of carp virus, nucleic acid of infectious hematopoietic necrosis virus, nucleic acid of viral haemorrhagic septicaemia virus, nucleic acid of infectious salmon anaemia virus,nucleic acid of salmonid alphavirus, and nucleic acid of infectious pancreatic necrosis virus. The upstream and downstream primers of the first primer pair to the sixth primer pair are sequentially the sequences as shown in Seq ID No.1 to Seq ID No.12. The reagent can carry out multiple detection on six salmonidae viruses, is simple and convenient to use, shortens the detection time of the six salmonidae viruses, improves the detection efficiency and quality, and has great significance for rapid detection and prevention and control of the six salmonidae viruses.

Description

technical field [0001] The application relates to the field of salmon trout virus detection, in particular to a reagent for multiple detection of salmon trout virus and its application. Background technique [0002] Salmon and trout are the general term for fish in the family Salmonidae, which includes all species of the subfamily Salmonidae and the subfamily Salmonidae. Salmon trout enjoys the reputation of "water ginseng" because of its tender meat, delicious taste and high nutritional value. It is a high-quality cultured species promoted by the Food and Agriculture Organization of the United Nations to countries all over the world. China began to introduce salmon and trout farming in the 1970s. At present, the salmon and trout farming area has reached more than 1,500 mu, and the annual production of salmon and trout is about 30,000 tons. It is one of the main cold-water fish farming species in China. [0003] In recent years, salmon and trout diseases have occurred frequ...

AI Technical Summary

Problems solved by technology

Among them, the virus isolation method has accurate detection results, but it takes a long time and requires high experimental conditions; ordinary PCR and real-time fluorescent PCR detection methods have greatly improved the sensitivity and accuracy of detection, but currently only work well for a single target. False-positive results are prone to occur in multi-target detection

Actual samples often have mixed infections of multiple viruses. Using single-target ordinary PCR or real-time fluorescent PCR detection methods to detect and analyze viruses one by one is not only time-consuming and laborious, but also not conducive to the rapid prevention and control of viruses.

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