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A kind of edible coating film and preparation method thereof

A technology of coating film and edible properties, applied in the field of edible coating film and its preparation, to achieve the effect of improving film-forming characteristics, compact spatial structure, and strong thermal stability

Active Publication Date: 2021-08-31
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the self-assembly behavior of collagen in the swim bladder of grass carp.

Method used

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  • A kind of edible coating film and preparation method thereof
  • A kind of edible coating film and preparation method thereof
  • A kind of edible coating film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of preparation method of edible coating film, comprises the following steps:

[0037] (1) Grass carp swim bladders were degreased and washed with water until neutral, added 0.5M acetic acid (1:10, w / v), 0.1% (w / v) 3000U / mg pepsin for 24 hours, centrifuged at 5000r / min for 20min, and Add pre-cooled sodium chloride to the supernatant for salting out, let it stand for 4 hours, then centrifuge to get the precipitate, repeat the salting out operation of the supernatant, dissolve the combined precipitate with 10 times the volume of 0.5% M acetic acid, dialyze, vacuum freeze-dry and store at -20°C Stand by to obtain the grass carp swim bladder collagen.

[0038] (2) After the grass carp meat is minced, add 10 times the volume of pre-cooled sodium chloride solution (50mmol / L) to homogenate, place in an ice bath and stir for 30min, filter with gauze, centrifuge at 8000r / min for 15min, take the precipitate and The grass carp myofibrillar protein was obtained by adding pre...

Embodiment 2

[0051] A kind of preparation method of edible coating film, comprises the following steps:

[0052] (1) After the grass carp swim bladder is degreased, it is fully washed with water until it becomes neutral, and 0.5M acetic acid (1:10, w / v), 0.1% (w / v) 3000U / mg pepsin is added to react for 36h, centrifuged at 5000r / min for 20min, and Add pre-cooled sodium chloride to the supernatant for salting out, let it stand for 4 hours, then centrifuge to get the precipitate, repeat the salting out operation of the supernatant, dissolve the combined precipitate with 10 times the volume of 0.5% M acetic acid, dialyze, vacuum freeze-dry and store at -20°C stand-by.

[0053] (2) Extraction of grass carp myofibrillar protein: After the grass carp meat is minced, add 10 times the volume of pre-cooled sodium chloride solution (60mmol / L) to homogenate, place it in an ice bath and stir for 30min, filter with gauze, 8000r / L Centrifuge at 1 min for 15 min, take the precipitate, add pre-cooled sodi...

Embodiment 3

[0066] A kind of preparation method of edible coating film, comprises the following steps:

[0067] (1) Grass carp swim bladders were degreased and washed with water until neutral, added 0.5M acetic acid (1:10, w / v) and 0.1% (w / v) 3000U / mg pepsin to react for 48h, centrifuged at 5000r / min for 20min, and Add pre-cooled sodium chloride to the supernatant for salting out, let it stand for 4 hours, and then centrifuge to collect the precipitate. use.

[0068] (2) Extraction of grass carp myofibrillar protein: After the grass carp meat is minced, add 10 times the volume of pre-cooled sodium chloride solution (70mmol / L) to homogenate, place it in an ice bath and stir for 30min, filter with gauze, 8000r / L Centrifuge at 1 min for 15 min, take the precipitate, add pre-cooled sodium chloride solution to homogenate, centrifuge, and cycle wash 3 times, the obtained precipitate is grass carp myofibrillar protein.

[0069] (3) Enzymatic hydrolysis of grass carp myofibrillar protein: Take ...

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Abstract

The invention discloses an edible coating film and a preparation method thereof, comprising the following steps: self-assemble collagen of grass carp swim bladder to obtain self-assembled collagen; configure self-assembled collagen to 4-8% (w / v) Dissolve the collagen solution in a water bath at 40-60°C for 20-40 minutes, add other coating materials, stir after mixing, cross-link at 50-65°C for 20-35 minutes, cool to room temperature, vacuum degassing, and naturally After drying, the film is peeled off to obtain an edible coating film. The invention utilizes the enzymatic hydrolyzate of grass carp myofibrillar protein to promote the self-assembly of grass carp swim bladder collagen, making its spatial structure more dense, improving the film-forming properties of grass carp swim bladder collagen, and the edible coating prepared by self-assembled grass carp swim bladder collagen The film has stronger thermal stability, mechanical properties, water resistance and emulsification.

Description

technical field [0001] The invention belongs to the technical field of biological food, and in particular relates to an edible coating film and a preparation method thereof. Background technique [0002] Grass carp is the first of the four major fish in my country, and is widely welcomed because of its advantages of high protein, low fat, and large catch. However, in the use and processing of grass carp, due to the relatively backward processing technology and eating habits, the swim bladder, fish skin, fish scales, etc. have not been fully utilized and are often discarded as leftovers. Extracting collagen from grass carp swim bladder not only reduces waste of resources but also increases economic benefits. [0003] Collagen is a kind of fibrous structural protein that widely exists in organisms. According to the difference of its molecular structure, it can be divided into various types such as I, II, and III, among which type I collagen is the most abundant in organisms. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08L5/08C08K5/053C08J5/18C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
CPCC07K14/78C08J5/18C08J2389/00C08J2405/08C08K5/053C12P21/06
Inventor 赵丹丹胡俊陈文烜
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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