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Liquid chromatography-mass spectrometry combined analysis method and interface device thereof

A technology of liquid chromatography mass spectrometry and analysis method, which is applied in the field of analysis methods combined with liquid chromatography mass spectrometry and an interface device thereof, can solve the problems of affecting signal intensity, reducing signal intensity, breaking the dynamic balance of mass spectrometry, etc., so as to improve ionization The effect of improving efficiency and signal strength

Active Publication Date: 2019-11-15
哈尔滨阿斯顿仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is also a mismatch between liquid chromatography and electrospray ionization mass spectrometry, which leads to the following shortcomings in the current LC-MS method:
The pure electrospray droplet diameter is 1-2μm, and the pneumatic-assisted electrospray droplet increases to 10μm under the condition of high flow rate, which leads to poor gasification effect and weak mass spectrometry signal
In addition, if the flow rate of the sample solution is too fast, the number of charges per unit volume of the sample solution will decrease, the ionization efficiency will deteriorate, and the signal intensity will also be affected.
[0004] ② Electrospray conditions are difficult to achieve optimal, thus affecting the signal intensity
That is to say, during the whole analysis process, the composition of the mobile phase is always changing, while other conditions of the mass spectrometer cannot be changed, which will break the dynamic balance of the parameters of the mass spectrometer, thereby reducing the signal intensity

Method used

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  • Liquid chromatography-mass spectrometry combined analysis method and interface device thereof
  • Liquid chromatography-mass spectrometry combined analysis method and interface device thereof
  • Liquid chromatography-mass spectrometry combined analysis method and interface device thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0060] refer to figure 1 , a liquid chromatography mass spectrometry analysis method, comprising the steps of:

[0061] S1, fix the tail of the probe sleeve 43 on the head of the probe base 4, then place the solid probe 2 inside the probe sleeve 43, and fix the tail of the solid probe 2 on the probe base On the head of the seat 4, the solid probe 2 points to the inlet 1 of the mass spectrometer and the tip 21 of the solid probe 2 has a certain distance from the inlet 1 of the mass spectrometer;

[0062] S2, the shunt 22 is connected to the solid probe 2 after passing through the hole on the probe sleeve 43, the shunt 22 points downward, and the connection between the shunt 22 and the solid probe 2 is 21 m away from the tip 21 of the solid probe 2 a certain distance;

[0063] S3, one end of the sample tube 3 passes through the hole on the probe casing 43 and is placed at the junction of the flow divider 22 and the solid probe 2, and the other end is connected to the outlet of...

Embodiment 2

[0070] On the basis of Example 1, the probe base 4 in S1 is a hollow structure and its tail is connected to the gas pipeline 42. The head of the probe base 4 is provided with a circle around the tail of the solid probe 2. The air holes 41 distributed in a circular array, the probe casing 43 fixed on the head of the probe base 4 contain the air holes 41 and the solid probe 2 inside the probe casing 43, and the dry gas can pass through the gas pipeline 42, the probe The base 4 and the air hole 41 are blown out along the direction of the solid probe 2 inside the probe casing 43 .

Embodiment 3

[0072] On the basis of Embodiment 1 or 2, in S5, while applying a high voltage on the solid probe 2, turn on the gas switch on the probe base 4, start to fill the gas pipeline 42 with dry gas, and the dry gas passes through The air hole 41 is blown out along the direction of the solid probe 2 inside the probe sleeve 43, assisting the desolvation of the electrospray droplets between the tip 21 of the solid probe 2 and the inlet 1 of the mass spectrometer in S7.

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Abstract

The invention relates to a liquid chromatography-mass spectrometry combined analysis method and an interface device thereof. A mass spectrometer performs analysis and obtains an analysis map through the following steps: installing a solid probe; setting a shunt piece; installing a sample tube; turning on a liquid chromatography system and the mass spectrometer and applying a high voltage to the solid probe; then injecting a sample solution to perform an electrospray process; and adjusting a position of the solid probe to obtain a maximum signal of the mass spectrometer. According to the invention, the solid probe with a shunt piece is used as a liquid chromatography-mass spectrometry combined electrospray ion source interface, and most of the sample effluent of the liquid chromatography system is shunted along the shunt piece and only a small fraction is sprayed. The spray droplet produced by the invention has small diameter and high surface charge density; therefore, the signal intensity of the invention is higher than that of the conventional liquid chromatography-mass spectrometry combined system.

Description

technical field [0001] The invention relates to the field of liquid chromatography mass spectrometry analysis, in particular to an analysis method of liquid chromatography mass spectrometry and an interface device thereof. Background technique [0002] Liquid chromatography (HPLC) methods can separate a variety of compounds, especially for thermally unstable and non-volatile samples that cannot be handled by gas chromatography. Electrospray ionization mass spectrometry (ESI-MS), as one of the most widely used mass spectrometry techniques, can analyze various types of compounds, especially the analysis of thermally unstable and non-volatile biomacromolecules. The ion source of electrospray mass spectrometry adopts a capillary tube with high voltage applied. The sample solution carried in the capillary tube forms a spray at the capillary mouth under the action of high voltage, and finally the formed ions enter the mass spectrometer to be detected. Therefore, the electrospray ...

Claims

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Application Information

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IPC IPC(8): G01N30/72H01J49/00H01J49/04
CPCG01N30/7266H01J49/0031H01J49/0431
Inventor 许庆轩窦建鹏卢佳杰林佳娣
Owner 哈尔滨阿斯顿仪器有限公司
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