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Method of isolating lipids from a lipids containing biomass

A technology of biomass and physical methods, applied in the direction of fat oil/fat production, fat production, food science, etc., can solve the problems of degreasing, not very sustainable, rapid corrosion of steel equipment, etc.

Pending Publication Date: 2019-11-15
EVONIK OPERATIONS GMBH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the use of large amounts of sodium chloride results in a defatted biomass by-product which cannot be used as a feed ingredient due to the high salt content, so the method is not very sustainable
Furthermore, the high salt concentration leads to rapid corrosion of the steel equipment used

Method used

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  • Method of isolating lipids from a lipids containing biomass
  • Method of isolating lipids from a lipids containing biomass
  • Method of isolating lipids from a lipids containing biomass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] Example 1: Preparation of suspension for demulsification test

[0120] Unwashed cell culture broth containing microbial cells (Schichychytrium sp.) with a biomass density exceeding 100 g / l was heated to 60° C. in a stirred vessel. After heating the suspension, the pH was adjusted to 7.5 using caustic soda (50 wt% NaOH solution), and then alkaline protease ( 2.4 FG (Novozymes)). Stirring was continued for 3 hours at 60°C. Afterwards, the lysed cell mixture was transferred to a forced circulation evaporator (GEA, Germany) and heated to 85°C. The mixture was concentrated in a forced circulation evaporator until a total dry matter content of about 30% by weight was reached.

Embodiment 2

[0121] Example 2: Effect of the amount of added base equivalents on oil release

[0122]To test the importance of the amount of alkali equivalents added on the efficiency of oil release from biomass, the effect of adding different amounts of caustic soda to biomass on oil release was tested. Table 1 describes the ratio of alkali equivalents added to total dry matter and the amount of oil released by the addition of caustic soda. use a mixing container B-DCU–Quad 2L (Sartorius, Germany), 1 L of enzyme-treated and subsequently concentrated broth was used for all experiments. The sample had a total dry matter of 30 wt%. Demulsification was carried out at a temperature of 80° C. for 24 hours. The suspension was stirred at 300 rpm. Add caustic soda in one go at the beginning. After 24 hours, the demulsified composition was neutralized to pH 7.5. After neutralization, a 50 g sample of the homogenized suspension was taken and the cell debris was separated by centrifugation at ...

Embodiment 3

[0126] Example 3: Effect of the amount of added base equivalents on oil release

[0127] To further test the importance of the amount of alkali equivalents added on the efficiency of oil release from the biomass, the effect of adding different amounts of caustic soda to the biomass on oil release was tested. Table 2 describes the ratio of alkali equivalents added to total dry matter and the amount of oil released by caustic soda addition. use a mixing container B-DCU–Quad 2L (Sartorius, Germany), 1 L of enzyme-treated and subsequently concentrated broth was used for all experiments. The sample had a total dry matter of 30.5 wt%. Demulsification was carried out at a temperature of 80° C. for 24 hours. The suspension was stirred at 300 rpm. Caustic soda was added gradually in three batches to keep the pH low. After 24 hours, the demulsified composition was neutralized to pH 7.5. After neutralization, a 50 g sample of the homogenized suspension was taken and the cell debri...

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Abstract

The current invention relates to a method of isolated polyunsaturated fatty acids containing lipids from a lipids containing biomass.

Description

technical field [0001] The present invention relates to methods for isolating lipids containing polyunsaturated fatty acids from lipid-containing biomass. Background technique [0002] Lipids containing PUFAs (polyunsaturated fatty acids) are of high importance in the feed, food and pharmaceutical industries. Due to overfishing, there is a high demand for alternative sources of lipids containing PUFAs other than fish oil. The results showed that, in addition to certain strains of yeast and algae, especially microalgal cells such as those of the order Thraustochytriales were very good sources of lipids containing PUFAs. [0003] But for microbial organisms, especially cells of the Thraustochytrides that produce lipids containing PUFAs, isolating the oil from said cells is a particular problem. The most efficient way to separate the oil is to use an organic solvent such as hexane. But the use of organic solvents leads to hazardous operating conditions, requires the use of e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/12A23D9/02
CPCA23D9/02C11B1/10C11B1/12C12P7/64C12P7/6463
Inventor M·迪尔X·D·董A·哈特曼M·海宁M·B·约翰逊J·莱贝特N·F·莱宁格尔K·L·老马修斯M·E·内贾科二世H·普法伊费尔C·拉贝S·E·E·里索G·M·尚克V·塔尔韦德D·A·廷斯利
Owner EVONIK OPERATIONS GMBH
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