Modified collagen based soft tissue adhesive and preparation method thereof

A soft tissue and adhesive technology, applied in surgical adhesives, applications, medical science, etc., can solve the problems of easy precipitation and high price of dopamine, and achieve the effect of good solubility and good adhesive performance

Active Publication Date: 2019-11-19
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, collagen only exists in the blend as an auxiliary component, and is not an effective component for bonding (the effective bonding component is actually the reaction product of oxidized sodium alginate and dopamine); in addition, the unmodified collagen is in Precipitation is easy to occur in the solution neutralized to pH 5-8, and the dopamine in the adhesive is expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Collagen extraction: collect collagen-rich leftovers in the tanning preparation section, grind and clean them, and then fully deash them. Use the acetic acid-pepsin combination method, and extract by shaking at a temperature not higher than 10°C. After centrifugation, salting-out, dissolution, dialysis and freeze-drying, the refined natural skin collagen is obtained.

[0027] Mix 1 g of gallic acid, 0.6 g of EDC, and 0.6 g of NHS into anhydrous DMF, and react under nitrogen protection. After reacting for 3 hours, a large amount of dichloromethane solution was added to the reaction solution, and the GA-NHS ester was gradually precipitated from the mixed solution. The supernatant was removed by centrifugation, and the remaining precipitate was repeatedly washed three times and vacuum-dried to obtain refined gallic acid-NHS ester.

[0028] Under the protection of nitrogen, an excess of gallic acid-NHS ester was added to the 5 mg / mL collagen solution (the solid content ra...

Embodiment 2

[0034] Collagen extraction: with embodiment 1.

[0035] Mix 1 g of gallic acid, 1.2 g of EDC, and 0.3 g of NHS into anhydrous DMF, and react under nitrogen protection. After reacting for 1 hour, a large amount of dichloromethane solution was added to the reaction solution, and the GA-NHS ester gradually precipitated from the mixed solution. The supernatant was removed by centrifugation, and the remaining precipitate was repeatedly washed three times and vacuum-dried to obtain refined gallic acid-NHS ester.

[0036] Under nitrogen protection, excess gallic acid-NHS ester was added to 10 mg / mL collagen solution (the solid content ratio of collagen to gallic acid-NHS ester was 2.4:1), so that the collagen amino group fully reacted with gallic acid-NHS ester for 5 hours to obtain Amino blocked collagen solution.

[0037] Under nitrogen protection, 0.1 g of polylysine and 0.35 g of gallic acid were dissolved in MES buffer (0.05 mol / L, pH=5.5). After E-PL and GA were completely d...

Embodiment 3

[0042] Collagen extraction: with embodiment 1.

[0043] Mix 1 g of gallic acid, 2.4 g of EDC, and 1.2 g of NHS into anhydrous DMF, and react under nitrogen protection. After reacting for 5 hours, a large amount of dichloromethane solution was added to the reaction solution, and the GA-NHS ester was gradually precipitated from the mixed solution. The supernatant was removed by centrifugation, and the remaining precipitate was repeatedly washed three times and vacuum-dried to obtain the refined gallic acid-NHS active ester.

[0044] Under the protection of nitrogen, add excess gallic acid-NHS ester to 1 mg / mL collagen solution (the solid content ratio of collagen and gallic acid-NHS ester is 4.8:1), so that the collagen amino group can fully react with gallic acid-NHS ester for 2.5 hours, A collagen solution with amino groups blocked is obtained.

[0045] Under nitrogen protection, 0.1 g of polylysine and 0.5 g of gallic acid were dissolved in MES buffer. After E-PL and GA we...

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PUM

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Abstract

The invention belongs to the field of collagen materials and particularly relates to a modified collagen based soft tissue adhesive and a preparation method thereof. The modified collagen based soft tissue adhesive is prepared from the following raw materials including collagen, polylysine, gallic acid and an oxidizing agent. By using active ingredients, including the polylysine and the gallic acid, with a source of biology and low cost for body chemical modification, the modified collagen has a good adhesion effect. The modified collagen provided by the invention has a very good application potential in the field of medical soft tissue adhesives.

Description

technical field [0001] The invention belongs to the field of collagen materials, and in particular relates to a soft tissue adhesive based on modified collagen and a preparation method thereof. Background technique [0002] In the traditional medical field, sutures or staples are often used for sealing and repairing wounds of soft tissues such as skin, muscle and intestines. Although this method is effective, it is cumbersome and laborious, and it also introduces new injuries to bring additional pain to the patient. In contrast, soft tissue adhesives have many advantages, such as good sealing, less damage, hemostasis, loadable drugs, convenient use, short operation time, no need to remove, and shallow scabs, etc., and have broad application prospects. [0003] Collagen is the main structural protein of the extracellular matrix, the protein with the highest content in the organism, and widely exists in the human body and animals. As a natural polymer material with a wide ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/04A61L24/00
CPCA61L24/0015A61L24/046A61L24/102A61L2300/21A61L2300/214A61L2300/404A61L2300/45C08L77/04
Inventor 张敏阳奇利陈礼辉黄六莲吴慧苗庆显倪永浩
Owner FUJIAN AGRI & FORESTRY UNIV
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