Rapid breeding method of rieger begonia
A begonia, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of cultivation failure, cutting seedling aging, cutting aging and other problems, achieve improved reproductive efficiency, high seedling survival rate, and meet the market. effect of supply
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Embodiment 1
[0029] A method for rapid breeding of Rieger crabapple, comprising:
[0030] S11. Take the strong plants of Rieger Begonia for isolation and aseptic culture, regularly disinfect, take the young leaves as explants, and disinfect the explants;
[0031] S12. cutting the explant, cutting off the outer layer of the leaf edge of the explant, and then dividing it into a plurality of primary tissues, and inducing and culturing the primary adventitious buds until small buds appear on the edge of the primary tissue;
[0032] S13. Cut the primary tissue with small buds on the edge, separate the independent small buds, divide the separated independent small buds into multiple secondary tissues, and subculture the independent small buds and secondary tissues. Bud induction culture;
[0033] S14. Repeat S13 to obtain proliferating buds. The size of the proliferating buds is larger than that of independent small buds. The proliferating buds are cultivated for strong seedlings. After the bud...
Embodiment 2
[0037] The difference between Example 2 and Example 1 is that in the S11 step, strong plants of Rieger Begonia are taken for isolation and aseptic culture, and the plants are sprayed with chlorothalonil solution every 5 to 8 days. In the solution, chlorothalonil:solvent=1:5000; take young leaves as explants, and the young leaves are leaves that have just grown for 5 to 7 days and have not fully expanded; the explants are sterilized and dried, and the Said disinfection is to wash with sterile water for 2 to 5 times in a sterile environment, then dry until there are no obvious drops of water on the surface of the leaves, and then wash the leaves with sterile water for 5 times.
Embodiment 3
[0039] The difference between embodiment 3 and embodiment 1 is that in the step S12, the explant is cut, the outer layer of the leaf edge of the explant is cut off, and then divided into a plurality of primary tissues, the primary tissue is a square, The side length is 1cm; the first-generation adventitious bud induction culture is carried out on the first-generation tissue until small bud spots appear on the edge of the first-generation tissue; during the first-generation adventitious bud induction culture process, 2000Lux light is used for 20d, and the light is 6~10h per day, and the temperature is 25 ℃, humidity 50%.
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