Rapid breeding method of rieger begonia

A begonia, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of cultivation failure, cutting seedling aging, cutting aging and other problems, achieve improved reproductive efficiency, high seedling survival rate, and meet the market. effect of supply

Inactive Publication Date: 2019-11-22
广州市名卉景观科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The conventional propagation method of Rieger Begonia is cutting propagation. The cutting propagation method has always had problems such as species degradation, cutting aging, and cuttings are prone to virus transmission, which leads to the aging of cutting seedlings of Rieger Begonia grown from cuttings, and premature cuttings that do not reach the appropriate plant type. Flowering, and the cutting seedlings carry virus, which leads to cultivation failure

Method used

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  • Rapid breeding method of rieger begonia

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Experimental program
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Effect test

Embodiment 1

[0029] A method for rapid breeding of Rieger crabapple, comprising:

[0030] S11. Take the strong plants of Rieger Begonia for isolation and aseptic culture, regularly disinfect, take the young leaves as explants, and disinfect the explants;

[0031] S12. cutting the explant, cutting off the outer layer of the leaf edge of the explant, and then dividing it into a plurality of primary tissues, and inducing and culturing the primary adventitious buds until small buds appear on the edge of the primary tissue;

[0032] S13. Cut the primary tissue with small buds on the edge, separate the independent small buds, divide the separated independent small buds into multiple secondary tissues, and subculture the independent small buds and secondary tissues. Bud induction culture;

[0033] S14. Repeat S13 to obtain proliferating buds. The size of the proliferating buds is larger than that of independent small buds. The proliferating buds are cultivated for strong seedlings. After the bud...

Embodiment 2

[0037] The difference between Example 2 and Example 1 is that in the S11 step, strong plants of Rieger Begonia are taken for isolation and aseptic culture, and the plants are sprayed with chlorothalonil solution every 5 to 8 days. In the solution, chlorothalonil:solvent=1:5000; take young leaves as explants, and the young leaves are leaves that have just grown for 5 to 7 days and have not fully expanded; the explants are sterilized and dried, and the Said disinfection is to wash with sterile water for 2 to 5 times in a sterile environment, then dry until there are no obvious drops of water on the surface of the leaves, and then wash the leaves with sterile water for 5 times.

Embodiment 3

[0039] The difference between embodiment 3 and embodiment 1 is that in the step S12, the explant is cut, the outer layer of the leaf edge of the explant is cut off, and then divided into a plurality of primary tissues, the primary tissue is a square, The side length is 1cm; the first-generation adventitious bud induction culture is carried out on the first-generation tissue until small bud spots appear on the edge of the first-generation tissue; during the first-generation adventitious bud induction culture process, 2000Lux light is used for 20d, and the light is 6~10h per day, and the temperature is 25 ℃, humidity 50%.

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Abstract

The invention relates to the field of plant cultivation, and provides a rapid breeding method of rieger begonia. The method is used for improving the breeding efficiency of the rieger begonia. The provided rapid breeding method of the rieger begonia comprises the steps that S11, young and tender leaves of healthy and strong plants of the rieger begonia are taken as explants; S12, the explants arecut to be divided into multiple primary tissues, and primary adventitious bud induction culture is performed on the primary tissues; S13, the primary tissues with budlet points occurring at the edgesare cut to separate the independent budlets, multiple subculture tissues are also obtained, and subculture adventitious bud induction culture is performed on the independent budlets and the subculturetissues; S14, S13 is repeated to obtain proliferative buds, seedling strengthening culture is performed on the proliferative buds, and the bud bodies are cut after growing to a certain size to obtainrooting seedlings; S15, rooting culture is performed on the rotting seedlings to obtain seedlings of the rieger begonia. In the culture process, and the survival rate of the seedlings is high, more rieger begonia plants can be obtained within the same culture time, so that the breeding efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of plant planting, in particular to a method for rapid breeding of Rieger crabapple. Background technique [0002] Rieger Begonia (scientific name: Rieger Begonias.) is Begoniaceae, Begonia genus. It is a group of winter-flowering hybrids obtained by crossing winter-flowering Soko begonias with many bulbous begonias. These hybrids combine the characteristics of short-day flowering of Soko begonias with the advantages of large and colorful flowers of bulbous begonias. They have the characteristics of winter flowering and many flowers, large flowers, disease resistance, no tuber formation and obvious dormancy, and are rare. Greenhouse potted flowers, very suitable for potted ornamental. Rieger Begonia has a very long flowering period, which can last from December to April of the next year. The flowering period is just during the Spring Festival. The flowers are diverse, colorful, and very beautiful. It has become an in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 江碧玉阮燕珠王玮马文卿沈荔荔阳雪修
Owner 广州市名卉景观科技发展有限公司
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