A rapid and efficient method for inducing the rooting of Binmei tissue culture seedlings

A technique for tissue culture seedlings and induction medium, applied in the field of botany, can solve the problems of shortening the cultivation period of Binmei tissue culture seedlings, less rooting and branches, and difficulty in cleaning the root medium, so as to shorten the cultivation period and keep the seedlings tidy and robust. , Improve the effect of rooting induction

Inactive Publication Date: 2017-11-07
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004]Technical problem: In order to overcome the technical problems of less rooting branches, difficult cleaning of root culture medium, and low seedling rate of transplanting in the existing rooting method of berry tissue culture seedlings Insufficient, the present invention provides a kind of rooting medium and the cultivation method that can effectively improve the rooting rate and the transplanting survival rate of the berry tissue culture seedling according to the growth characteristics of the berry, so as to improve the rooting rate and the transplanting survival rate of the berry tissue culture seedling , and the method of cultivating robust seedlings has the characteristics of cost saving and high efficiency, shortening the cultivation cycle of hamster tissue culture seedlings, etc., and provides a method for rapid production of high-quality seedlings for large-scale production

Method used

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  • A rapid and efficient method for inducing the rooting of Binmei tissue culture seedlings
  • A rapid and efficient method for inducing the rooting of Binmei tissue culture seedlings
  • A rapid and efficient method for inducing the rooting of Binmei tissue culture seedlings

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: the impact of basal culture medium on the rooting of berry tissue culture seedlings

[0024] Cut 3-5cm rootless tissue-cultured seedlings of Lime plum, inoculate them into basic medium + 0.3mg / L NAA + 3.0g / L activated carbon + 5.6g / L agar + 20g / L sucrose, and the pH value is 5.8. Inoculate 4 plants per bottle, 20 plants per treatment, repeat 3 times, observe once every 7 days, and count the results after 20 days. As can be seen from Table 1, the 3 kinds of basic medium have a more significant effect on the rooting of the berry tissue culture seedlings, and the effect of using 1 / 2WPM as the basic medium is the worst, no matter the number of roots, root length or rooting rate are all low , and the plant growth is not good, but the rooting effect of BPM as the basic medium is the best, not only the number of roots is large, but the rooting rate is 100%, and its higher transplanting survival rate is also verified in the later transplanting test , Therefore, B...

Embodiment 2

[0027] Embodiment 2: The influence of NAA concentration on the rooting of Lime plum tissue culture seedlings

[0028] Cut 3-5cm rootless tissue-cultured seedlings of Lime plum, inoculate them into BPM+0-0.7mg / L NAA+3.0g / L activated carbon+5.6g / L agar+20g / L sucrose, and the pH value is 5.8. Inoculate 4 plants per bottle, 20 plants per treatment, repeat 3 times, observe once every 7 days, and count the results after 20 days. It can be seen from Table 2 that the rooting rate can reach 85.0% when NAA is not added, but the average root length is low, and the growth of the aboveground part of the plant is general, which is not conducive to the subsequent transplanting of continuous seedlings; when the concentration of NAA is added The rooting rate and the average root length were significantly increased at 0.1, but when the concentration of NAA was added to 0.7mg / L, there was no significant difference in other indicators except that the average root length decreased slightly (but th...

Embodiment 3

[0031] Embodiment 3: the influence of activated carbon concentration on the rooting of Lime plum tissue culture seedlings

[0032] Cut 3-5cm rootless tissue-cultured seedlings of Lime plum, inoculate them into BPM+0.3mg / L NAA+0-4.0g / L activated carbon+5.6g / L agar+20g / L sucrose, and the pH value is 5.8. Inoculate 4 plants per bottle, 20 plants per treatment, repeat 3 times, observe once every 7 days, and count the results after 20 days. As can be seen from Table 3, when no activated carbon was added, the rooting rate of the tissue-cultured seedlings of Lime plum was very low, only 70.0%, and the bottom was easy to grow callus, which was not conducive to the growth of the aboveground part, so the growth of the plant was poor; When the addition amount of activated carbon is 1.0g / L, although the rooting rate and number of roots have increased, the growth of the plant is still not very good; The roots sprouted, and the rooting rate reached 100% in 14 days, and the number of roots,...

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Abstract

The invention discloses a rooting basal medium (BPM) and a cultivation method thereof, characterized in that the components of the MS basal medium are improved, rooting inducers (NAA, activated carbon) are added, and culture conditions are adjusted. , the rooting rate in the bottle can reach 100% in 14 days, so that the transplanting survival rate can reach more than 90%, which is 12%~34% higher than the current report. It only takes 45 days from inoculation to transplanting to survive, which not only improves the rooting efficiency of the tissue-cultured seedlings of Lime plum, but also has the characteristics of neat and robust seedlings, saving costs and high efficiency, and shortening the cultivation cycle of tissue-cultured seedlings of Lime plum. Chemical production provides the means to rapidly produce high-quality seedlings.

Description

technical field [0001] The invention belongs to the technical field of botany, and in particular relates to a method for inducing the rooting of the tissue-cultured seedlings of Lime plum. Background technique [0002] Prunus maritima Marshall (Prunus maritima Marshall) belongs to the genus Prunus in the family Rosaceae. It is a sandy beach shrub-like fruit tree distributed along the North Atlantic coast from Newfoundland to North Carolina (Clark, 2000). The fruit of berry is sweet and sour, and besides being eaten fresh, it can also be used for the processing of a series of fruits such as jam, fruit juice, fruit wine, jelly, and soft candy. Binmei has strong adaptability and can survive and bear fruit in various adversities. It is a tree with important economic value and ecological value. [0003] The propagation methods of Lime plum include seed propagation, root tiller propagation, cutting propagation and tissue culture rapid propagation. The reported survival rate of se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王小敏朱泓吴文龙李维林胡慧敏
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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