Urine flow cytometry as biomarker of renal diseases

A cell and kidney disease technology, applied in the field of urine flow cytometry as a biomarker of kidney disease, can solve the problems of potential disease recurrence, chronic transplant injury, transplant rejection, etc., and achieve the effect of reducing the number

Inactive Publication Date: 2019-11-26
CHARITE UNIVS MEDIZIN BERLIN
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Problems solved by technology

However, every transplant carries the risk of graft rejection, recurrence of underlying disease, or chronic graft injury

Method used

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  • Urine flow cytometry as biomarker of renal diseases
  • Urine flow cytometry as biomarker of renal diseases
  • Urine flow cytometry as biomarker of renal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0166] example

[0167] 1. Method

[0168] sample collection

[0169] Urine samples from patients undergoing kidney transplantation were collected by catheter or spontaneously. Store samples in sterile beakers and be ready for analysis within 6 hours of collection.

[0170] cell separation

[0171] Samples were dispensed in 50 ml tubes and centrifuged at 4 °C and 1300 g for 8 min. The supernatant was discarded and the pellets were resuspended and combined in PBS / BSA buffer to give 40ml per sample. Unfixed cells (T cells) were analyzed on the day of sample collection. Fixed cells were analyzed within 7 days of sample collection.

[0172] Fixed cells: For staining for intracellular markers, transfer 10 ml of detached cells to a 15 ml tube and centrifuge at 1300 g for 8 min at 4 °C. Discard the supernatant and resuspend the pellet in PBS / BSA buffer. The solution was transferred to a 1.5 ml tube and centrifuged at 4 °C and 2300 g for 8 min. Discard the supernatant. T...

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Abstract

The invention provides a method of assigning to a patient a likelihood of having a kidney disease, or a likelihood of undergoing kidney transplant rejection, comprising the steps of providing a urinesample from the patient and determining the concentration of T cells, podocytes and proximal tubular epithelial cells. The ratios of these cell types are used for determining the risk of a kidney disease or transplant rejection.

Description

technical field [0001] The present invention relates to the detection of kidney disease and renal transplantation rejection by analyzing the concentrations and ratios of T cells, podocytes and renal tubular epithelial cells in urine samples. Background technique [0002] Currently, kidney disease is primarily diagnosed by assessing the glomerular filtration rate (creatinine clearance), analyzing blood-urine barrier permeability (proteinuria), and microscopic analysis of cells present in the urine. Microscopic analysis is a semiquantitative examination of normally unstained cells and is highly examiner dependent. Current non-invasive methods cannot guarantee high sensitivity and specificity. [0003] Kidney transplantation is the best therapy for patients with end-stage renal insufficiency. However, every transplant carries the risk of graft rejection, recurrence of the underlying disease, or chronic graft injury. If impaired or deteriorating graft function is detected in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/505G01N33/5091G01N2800/245G01N2800/347G01N2800/52G01N15/14G01N33/493
Inventor 菲利普·英格尼娜·格里克汉娜·安东尼娅·布兰德瓦莱丽·朗汉斯佩特拉·雷因克
Owner CHARITE UNIVS MEDIZIN BERLIN
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