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A method for disinfection of explants with bud stems of black tiger and its method for directly inducing the rapid proliferation of sterile buds

A disinfection method and explant technology, applied in botany equipment and methods, horticultural methods, planting substrates, etc., can solve the problems of easy occurrence of endophytes and difficult sterilization

Active Publication Date: 2021-01-12
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The primary purpose of the present invention is to provide a method for disinfection of the explants of the black tiger with bud stem section, which is difficult to sterilize and prone to the occurrence of endophytic bacteria

Method used

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  • A method for disinfection of explants with bud stems of black tiger and its method for directly inducing the rapid proliferation of sterile buds
  • A method for disinfection of explants with bud stems of black tiger and its method for directly inducing the rapid proliferation of sterile buds
  • A method for disinfection of explants with bud stems of black tiger and its method for directly inducing the rapid proliferation of sterile buds

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Experimental program
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Embodiment 1

[0036] Do the following in order:

[0037] 1. Transplanting field materials: From January to April every year, transplant black tigers that are 2-3 years old in field cultivation into plastic pots with a height of 30 cm and a diameter of 25 cm, cut off the stems and leaves of the above-ground part and water them After 3-5 months of normal water and fertilizer management in the roof greenhouse of the tree building laboratory of Central South University of Forestry and Technology, the germinated semi-lignified stem section with buds is used as the test material of the present invention.

[0038] 2. Explant treatment: cut the semi-lignified stems with buds into 3-4cm long stems, each stem has an axillary bud, put it in a beaker and wash it with detergent 1-2 times, and then use running water Rinse for 1 hour, rinse off the mucus secreted by the stem and set aside.

[0039]3. Disinfection of explants In the morning of sunny days from July to October 2018-2019, select semi-lignifi...

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Abstract

The invention provides a method for sterilizing explants of black tiger with bud stems and a method for directly inducing rapid proliferation of sterile buds. Including the selection, treatment and disinfection of explants, primary culture, subculture and other processes. The present invention overcomes the difficult problem of the initial culture of black tiger stem segment tissue culture, and the present invention has the advantages of low explant contamination rate, fast reproduction rate and robust axillary bud proliferation, and not only provides a new method for obtaining black tiger tissue culture aseptic proliferating buds Feasible technical approaches will provide preliminary technical support for the rapid propagation of black tigers through tissue culture and industrial seedlings in the future.

Description

technical field [0001] The invention belongs to the field of black tiger tissue culture, and in particular relates to a method for disinfecting explants of black tiger with bud stems and a method for directly inducing rapid proliferation of sterile buds. Background technique [0002] Black Tiger (Kadsura coccinea) is an evergreen vine belonging to the genus Schisandra in the family Magnoliaceae. The fruit is an aggregate fruit, the size of the fruit is unique like a football, the diameter of the fruit is 10-12cm, the weight of the fruit is about 600g, the large fruit reaches 1200g, the young fruit is green and dark red when mature. The pulp is like grapes, the flesh color is like lychee, and the fruit fragrance is like apple. It has good color, fragrance and taste. It is a third-generation new multi-purpose fruit tree that integrates food, medicine, greening and ornamental. It is urgent to be developed and has great development potential. Black tigers are distributed in Vi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/005A01H6/00A01H5/08A01G17/00A01G2/10A01G7/06A01G24/22A01G23/04
Inventor 李泽仲嘉玥刘洋王森包芬谭晓风曹丽仙马若楠杨奥
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY