Device and method for measuring cell mechanical properties

Abstract

The invention discloses a measuring device for cell mechanical properties, which includes a laser force measurement system, a microscopic observation system, a position control system, a signal receiving / converting and digital control system, a post-processing software system, and a liftable stage; The system includes a double-probe component, a double-probe component load-bearing pressure head, a laser light source and a position detector; the microscopic observation system includes a monocular microscope and a multi-light source component; the position control system includes a position detector displacement controller, a laser light source displacement control Device, laser force measuring system displacement controller, sample stage XY axis displacement controller, sample stage Z axis displacement controller and nanometer Z axis displacement controller; signal receiving / converting and digital control system includes stage controller, position detector A signal processing unit and a computer; the post-processing software system includes a sensitivity measurement module, a thermal curve analysis module, a force curve analysis module and a global image processing module.

Description

technical field [0001] The invention relates to the field of cell molecular signal interaction in biophysics, in particular to a measuring device and a measuring method for cell mechanical properties. Background technique [0002] Cell mechanical properties are mainly cell Young's modulus and cell surface adhesion properties. In cell mechanics, the Young's modulus of the cell reflects the stiffness of the cell. The stiffness of cells is related to many physiological and pathological states. Cell surface adhesion properties refer to the mechanical properties of the interaction between cell surface molecular receptors and extracellular ligands. Physiological and pathological states of organisms or changes in the microenvironment of cells may affect the adhesion characteristics of cell surfaces. In the physiological environment, there are usually multiple receptors on the cell surface, some receptors are independent of each other, some receptors interact with each other, and...

AI Technical Summary

Problems solved by technology

However, there are still disadvantages in this technique, namely, the small dynamic range of the measurement and the inability to measure the Young's modulus of cells

When measuring, it is necessary to use a micropipette to absorb cells or beads, and then perform a motion cycle of approach-contact-pull back. The force measurement range determined in this process cannot be too large, which limits the force measurement dynamics of this technology. range size (10 1 -10 3 pN)

In addition, the "probes" used in this technology are adsorbed cells or pellets, and a suitable cell model cannot be established, so it is also impossible to calculate a suitable cell Young's modulus result based on the force-distance curve

[0006] In addition, at present, whether it is commercial atomic force microscope or biofilm force probe technology, when measuring samples, only a specific size of petri dish can be selected, which is due to its compact structure design, which is largely limited the size of experimental samples

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