A dynamic cell culture method and culture device for simulating dynamic environment in vivo

Abstract

The invention relates to a dynamic cell culture method and a culture device for simulating a dynamic environment in the body. The culture method is as follows: the cells are adhered to a flexible base material for culture in vitro, and the flexible base material is applied with a certain frequency and direction during the culture process. For a certain cyclic dynamic load, the load gradually increases with time in each cycle, and then gradually decreases with time when it reaches the maximum value. The deformation rate of the flexible substrate changes synchronously with the load, first increases linearly, and then decreases linearly Small; the culture device includes a cell culture chamber, a clamping device for clamping a flexible substrate, an indenter for applying a load to the flexible substrate, and a device for driving the indenter to reciprocate in a direction perpendicular to the flexible substrate The centering slider crank mechanism, the clamping device and the pressure head are located in the cell culture chamber. The culture method of the invention can accurately simulate the interaction relationship of flexible implant-cells in the body; the culture device of the invention is easy to assemble, easy to use, and has great popularization value.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and relates to a dynamic cell culture method and a culture device for simulating a dynamic environment in a body. Background technique [0002] In vitro cell culture is of great significance for judging the growth and development of cells after material implantation in vivo, and the integration performance of materials and tissues. In vitro cell culture refers to the growth, development and migration of cells in an in vitro environment by simulating the in vivo environment. Compared with animal experiments and in vivo experiments, in vitro cell culture has the advantages of low cost, simple operation, easy observation and no ethical issues. However, there are still many shortcomings in in vitro cell culture. In vitro cell culture is mainly static culture, including culture bottle method, culture dish method, culture plate method, etc., which cannot accurately simulate the in vivo environmen...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that in vitro cell culture cannot accurately simulate the internal environment, especially the dynamic environment under different stress conditions in the prior art, and provide a dynamic cell culture method and a culture device for simulating the dynamic environment in the body, especially provide a An in vitro dynamic cell culture method and culture device that simulates the axial and radial mechanical stimulation of flexible substrates in vivo, the method can simulate the in vivo environment during in vitro culture, so that cells can be cultured under conditions that are more suitable for the in vivo environment , the data obtained are closer to the actual in vivo situation. Compared with in vivo experiments, the experimental cost of this method is greatly reduced, and the culture device used in this method is easy to operate, and different parameters can be set according to needs.

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