Application of IL-6 in preparation of drug for treating lumbar disc herniation

A lumbar intervertebral disc herniation and IL-6 technology, applied in the field of medical research, can solve the problems of abnormal cell transcription and expression, affecting disease susceptibility, and increasing the risk of lumbar intervertebral disc herniation.

Pending Publication Date: 2019-12-20
内蒙古医科大学第二附属医院
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Problems solved by technology

Professor Zhu Yong's team found that genetic variation in the IL-6 promoter region caused by IL-6 gene polymorphisms (rs1800796, rs1524107, rs2069840) in the Han population may be associated with an increased risk of lumbar disc herniation , and lead to abnormal cellular transcription and expression, thereby affecting individual susceptibility to various diseases
Nowadays, the higher expression level of IL-6 is often used as the clinical diagnosis basis for lumbar disc herniation, and the application of IL-6 in the preparation of drugs for the treatment of lumbar disc herniation has not yet been reported.

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  • Application of IL-6 in preparation of drug for treating lumbar disc herniation
  • Application of IL-6 in preparation of drug for treating lumbar disc herniation
  • Application of IL-6 in preparation of drug for treating lumbar disc herniation

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Embodiment Construction

[0017] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments:

[0018] 1. Main materials and reagents

[0019] DMEM / F12, trypsin, fetal bovine serum (Gibico, USA); Trizol (Ambion, USA); reverse transcription kit (Termo, USA) qPCR kit (KAPA, Switzerland); type II collagenase (Biofoxx, Sweden) ); mouse anti-Smad2, rabbit anti-Smad7, mouse anti-p-STAT3, rabbit anti-TSLP, HRP-labeled secondary antibodies (Shanghai Sangon Bioengineering Co., Ltd., Shanghai, China); culture flasks, culture plates (NEST , Thermo, USA).

[0020] 2. Isolation and culture of rat nucleus pulposus cells

[0021] 4-week-old female SD rats (provided by the Animal Experiment Center of Xi'an Jiaotong University) were sacrificed by excessive intraperitoneal injection of anesthesia, and soaked in 75% ethanol for 10 minutes. Separate the spine from the back on the sterile laboratory table, peel off the a...

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Abstract

The invention discloses application of IL-6 in preparation of drug for treating lumbar disc herniation. The IL-6 regulates expression of TSLP by a JAK/STAT signal path, and the TSLP can promote infiltration of macrophages to intervertebral disc tissue by inducing expression increase of monoeyte chemotactic protein-1 (MCP-1) so as to promote the re-absorption process of the intervertebral disc tissue.

Description

technical field [0001] The invention relates to the field of medical research, in particular to the application of IL-6 in the preparation of medicines for treating lumbar intervertebral disc herniation. Background technique [0002] Lumbar intervertebral disc herniation refers to the degeneration of lumbar intervertebral disc or fiber rupture caused by trauma, which causes the nucleus pulposus to rupture and protrude from the intervertebral disc, compressing the lumbar nerves, and resulting in radiating pain. This is called lumbar disc herniation. Currently, only a small number of patients with herniated disc herniation and lumbar disc herniation whose symptoms have not been relieved by conservative treatment receive surgical treatment. Studies by Yu Zhenhan and others have shown that herniated intervertebral disc tissue can achieve self-healing through the reabsorption process, but the underlying mechanism is still unclear. Wang Zhiwei and Yuan Hu proposed that macrophage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/20A61P19/08A61P29/00
CPCA61K38/204A61P19/08A61P29/00
Inventor 祝勇杨学军达逸峰黄智王文磊李莉
Owner 内蒙古医科大学第二附属医院
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