Method for extracting superoxide dismutase from plants as raw material

A technology of superoxide and plant raw materials, applied in the biological field, can solve the problems of complicated separation and extraction process, repeated addition and repeated separation, chemical pollution, etc., and achieves the effect of long activity retention time, good quality and high activity.

Pending Publication Date: 2019-12-27
王喜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Patent application number 97108883 discloses a method for extracting SOD from earthworms. The steps are: washing worms, purifying, infiltrating the solution to prepare SOD mother liquor, coarse filtration, centrifuging to obtain supernatant, ultrafiltration, reverse osmosis concentration, freezing Drying etc., patent application number 86100551 discloses a kind of purification method of superoxide dismutase (SOD), and it is that animal liver homogenate is heated step by step under the condition of bivalent copper salt and dialysis precipitation removes impurity, extracts SOD; Ammonium sulfate step-by-step salting out, removing miscellaneous eggs, collecting SOD-containing protein, and then dialysis, concentration, and drying, can obtain SOD with a specific activity of 2789 units / mg, but when separating and extracting SOD, it is necessary to add more The substance has been separated many times, which makes the separation and extraction process cumbersome and complicated, and is likely to cause chemical pollution. It can be seen that the implementation of the above scheme is difficult due to the defects of raw materials or methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for extracting superoxide dismutase from plants, the steps are as follows:

[0026] (1) Mix and pulverize golden okra, pine needles and cypress herb until the diameter is 100 meshes, then add an active protective agent of 0.02 times the mass of the mixture of golden okra, pine needles and cypress herb to carry out wall-breaking treatment to obtain a plant slurry for later use;

[0027] (2) Keep the plant slurry at a constant temperature of 50°C for 30 minutes to carry out active proliferation and fission;

[0028] (3) Centrifuging the plant slurry after active proliferation and fission, the centrifugal speed is 4000rpm, the centrifugation time is 30min, and the active liquid is obtained after removing the precipitate;

[0029] (4) Get equal amount of zinc gluconate and alkaline protease to mix, add concentration to wherein after adding the ethanol that concentration is 97 to the mass ratio of ethanol and equal mass zinc gluconate and alkaline protease mixture...

Embodiment 2

[0032] The method for extracting superoxide dismutase from plants, the steps are as follows:

[0033] (1) after the golden okra is crushed to a diameter of 80 meshes, an active protective agent of 0.005 times the quality of the golden okra is added for wall-breaking treatment to obtain a plant slurry for subsequent use;

[0034] (2) Keep the plant slurry at a constant temperature of 50°C for 25 minutes to carry out active proliferation and fission;

[0035] (3) Centrifuging the plant slurry after active proliferation and fission, the centrifugal speed is 4000rpm, the centrifugation time is 10min, and the active liquid is obtained after removing the precipitate;

[0036] (4) Get equal amount of zinc gluconate and alkaline protease to mix, add concentration to wherein after adding the ethanol that concentration is 95 to the mass ratio of ethanol and equal mass zinc gluconate and alkaline protease mixture with 60 times of zinc gluconate and alkaline protease mixture is 7: 3. Obt...

Embodiment 3

[0039] Vegetable ingredients are golden okra, sea buckthorn, pine needles, clover herb

[0040] The method for extracting superoxide dismutase from plants, the steps are as follows:

[0041] (1) Grinding golden okra and seabuckthorn to a diameter of 100 meshes, adding an active protective agent of 0.02 times the mass of the mixture of golden okra and seabuckthorn to carry out wall-breaking treatment to obtain a plant slurry for subsequent use;

[0042] (2) Keep the plant slurry at a constant temperature of 55°C for 35 minutes to carry out active proliferation and fission;

[0043] (3) centrifuging the plant slurry after active proliferation and fission, the centrifugal speed is 6000rpm, the centrifugation time is 30min, and the active liquid is obtained after removing the precipitate;

[0044] (4) Get equal amount of zinc gluconate and alkaline protease to mix, add concentration to wherein after adding the ethanol that concentration is 97 to the mass ratio of ethanol and equa...

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PUM

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Abstract

The invention discloses a method for extracting superoxide dismutase from plants as a raw material. By utilizing the characteristic of combining zinc and protease, the superoxide dismutase in plant wall broken active liquid is activated and extracted, and thus, ideal superoxide dismutase is obtained. According to the extraction method disclosed by the invention, the cost is low, the process is simple, the operation is convenient and practical, and the extracted superoxide dismutase is high in activity, good in quality and long in activity retention time and can be directly applied to filling raw materials of nutrients, healthcare products, drugs or foods.

Description

technical field [0001] The invention relates to a method for extracting superoxide dismutase, in particular to a method for extracting superoxide dismutase from plants, and belongs to the field of biotechnology. Background technique [0002] SOD is the abbreviation of superoxide dismutase (superoxide dismutase). The code of the International Enzyme Commission is EC 1.15.1.1. It belongs to redox enzymes. SOD enzyme is one of the important biological protection enzymes. Aging is an active substance that can remove excessive free radicals in the body. This substance is closely related to the aging of the body, tumor occurrence, autoimmune diseases and radiation protection. It is useful for the treatment of rheumatoid arthritis, cataracts, cystitis, skin diseases , lupus erythematosus and other diseases have better curative effect, SOD enzyme has no antigenicity, less adverse reactions, and less side effects. It is a medicinal enzyme with high medical value and broad prospects. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 王喜张玉芝
Owner 王喜
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