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Building method of pancreatic cancer ascites mouse animal model

A technology for establishing methods and animal models, which is applied in animal husbandry and other fields, and can solve problems such as complex operation, high difficulty, and low success rate of modeling

Inactive Publication Date: 2019-12-31
THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing methods for establishing animal models of pancreatic cancer ascites mice are orthotopic tumor transplantation, which is complicated and difficult to operate, and the success rate of modeling is low

Method used

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  • Building method of pancreatic cancer ascites mouse animal model
  • Building method of pancreatic cancer ascites mouse animal model
  • Building method of pancreatic cancer ascites mouse animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] Study on mouse animal model of pancreatic cancer ascites

[0018] 1. Recovery and culture of Panc02 cells

[0019] Preheat the water bath to 37°C, quickly take out the Panc02 cells from the liquid nitrogen tank, check whether the cryopreservation tube is well sealed, tighten the cap of the cryopreservation tube, and quickly place the cell cryopreservation tube in a 37°C water bath, shaking constantly Freeze the tube, let it be evenly heated and completely melt within 1 minute, observe the dissolution of the cell solution, when the cells in the tube are completely dissolved, quickly take out the tube, and wipe the outside of the tube with 75% alcohol, Move the cryovials to the ultra-clean cell bench. Transfer the cell suspension in the freezing tube into a 15ml centrifuge tube with a pipette, add PBS buffer to 10ml, and gently mix the cell suspension with a pipette. Tighten the cap of the centrifuge tube and place the centrifuge tube in the centrifuge. After balancing,...

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Abstract

The invention discloses a building method of a pancreatic cancer ascites mouse animal model. The method includes: using pancreatic cancer Panc02 cells for C57BL / 6 mouse intraperitoneal injection, extracting ascites directly for new-generation mouse intraperitoneal injection after ascites appears, repeating till tenth-generation mice, extracting pancreatic cancer cells in first-generation, fifth-generation and tenth-generation mouse ascites for purifying culture, and conducting in-vitro experiments and mouse in-vivo experiments on the pancreatic cancer cells after purifying culture to try to find out optimal cell amount and cell generation number for extraction for molding. Compared with existing nude mouse pancreatic cancer ascites models, the method has the advantages that a pancreatic cancer ascites model of autoimmune C57BL / 6 is created for the first time, and the method is simple and convenient in operation, short in modeling time and high in success rate. By the method, the mousepancreatic cancer ascites model can be built quickly and efficiently, and a proper carrier tool is provided for pathogenesis clarifying, diagnosing and treating of pancreatic cancer ascites.

Description

technical field [0001] The invention belongs to a method for establishing an animal model, in particular to a method for establishing a mouse animal model with ascites of pancreatic cancer. Background technique [0002] Experts predict that the death rate of pancreatic cancer will rise to the second place among all malignant tumors by 2030. Pancreatic cancer is prone to distant metastasis in the early stage of the disease. Metastasis to the peritoneum may be accompanied by symptoms such as malignant ascites and concurrent intestinal obstruction. Ascites in pancreatic cancer will seriously affect the quality of life of patients and shorten the survival time of patients. Therefore, clinical work The primary task in this study is to clarify the pathogenesis of pancreatic cancer ascites and to explore ways to inhibit pancreatic cancer ascites. [0003] At present, most mouse models of pancreatic cancer ascites are nude mouse pancreatic cancer ascites models lacking T cell immun...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0331
Inventor 覃山羽姜海行卢东诚覃沁怡胡榜利雷荣娥
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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