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Preparation method of composite microbial liquid with deodorization and epidemic prevention functions

A technology of complex microbial bacteria and functions, which is applied in the field of preparation of complex microbial bacterial liquid, can solve the problems of high environmental pressure, pollution of breeding waste, lack of efficient and safe biological deodorant, etc., and achieve the effect of eliminating odor and efficient growth and reproduction

Pending Publication Date: 2019-12-31
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Pigs have a long history of raising pigs in China. They are the breed of livestock and poultry with the widest range of breeding and the largest number of farming households. They occupy an important position in the structure of China's animal husbandry. Sewage, feces, stench, etc. cause serious environmental pollution. Especially in recent years, with the rapid development of my country's aquaculture industry, the problem of aquaculture waste pollution has become increasingly prominent. It has become the focus and hot issue of complaints from the public, and the pressure on environmental protection is increasing It also brings hidden dangers to the prevention and control of animal diseases and the quality and safety of farmed products.
According to statistics, the annual output of pig farm was

Method used

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  • Preparation method of composite microbial liquid with deodorization and epidemic prevention functions
  • Preparation method of composite microbial liquid with deodorization and epidemic prevention functions

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1

[0021] Reference figure 1 The method for preparing a composite microbial liquid with deodorizing and anti-epidemic functions proposed by the present invention includes the following steps:

[0022] S1. Medium burying: Choose deep mountain soil, remove the surface soil layer, put the medium dough into the soil, cover it, and cover a certain amount of pine leaves on its upper surface. The medium dough should be placed in a thick pine forest. The deep soil layer, the trench-shaped pit foundation with a depth of 25-30 cm, dug and buried by the medium dough;

[0023] S2, original seed fermentation: take corn starch 0.9%, glucose 0.8%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.2%, peptone 1.1%, yeast extract 1.2%, urea 0.3%, and make up the balance with sterile water , Ph is 2.0-4.5 to carry out the original seed fermentation of the medium dough in S1;

[0024] S3, raw material collection: 15 days later, a certain amount of medium dough is excavated and ret...

Example Embodiment

[0027] Example 2

[0028] Reference figure 1 The method for preparing a composite microbial liquid with deodorizing and anti-epidemic functions proposed by the present invention includes the following steps:

[0029] S1. Medium burying: Choose deep mountain soil, remove the surface soil layer, put the medium dough into the soil, cover it, and cover a certain amount of pine leaves on its upper surface. The medium dough should be placed in a thick pine forest. The deep soil layer, the trench-shaped pit foundation with a depth of 25-30 cm, dug and buried by the medium dough;

[0030] S2, original seed fermentation: take corn starch 0.9%, glucose 0.8%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.2%, peptone 1.1%, yeast extract 1.2%, urea 0.3%, and make up the balance with sterile water , Ph is 2.0-4.5 to carry out the original seed fermentation of the medium dough in S1;

[0031] S3, raw material collection: 15 days later, a certain amount of medium dough is excavated and ret...

Example Embodiment

[0034] Example 3

[0035] Reference figure 1 The method for preparing a composite microbial liquid with deodorizing and anti-epidemic functions proposed by the present invention includes the following steps:

[0036] S1. Medium burying: Choose deep mountain soil, remove the surface soil layer, put the medium dough into the soil, cover it, and cover a certain amount of pine leaves on its upper surface. The medium dough should be placed in a thick pine forest. The deep soil layer, the trench-shaped pit foundation with a depth of 25-30 cm, dug and buried by the medium dough;

[0037] S2, original seed fermentation: take corn starch 0.9%, glucose 0.8%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.2%, peptone 1.1%, yeast extract 1.2%, urea 0.3%, and make up the balance with sterile water , Ph is 2.0-4.5 to carry out the original seed fermentation of the medium dough in S1;

[0038] S3, raw material collection: 15 days later, a certain amount of medium dough is excavated and ret...

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Abstract

The invention discloses a preparation method of a composite microbial liquid with deodorization and epidemic prevention functions. The method comprises the following steps: S1, culture medium burying;S2, stock seed fermentation; S3, raw material collecting; S4, enrichment and domestication; and S5, expanding preparation of a microbial liquid: preparing a liquid culture medium obtained in the stepS4 from the following materials in percentage by weight: 0.9-2.0% of peptone, 0.1-1.0% of urea, 0.1% of sodium acetate, 0.8-2.0% of yeast extractum, 0.3% of monopotassium phosphate, 0.1% of ammoniumchloride, 0.1% of table salt, 0.02% of magnesium chloride hexahydrate, 0.7% of glucose and the balance of distilled water, setting the pH value to be 6-8, and performing enlarged culture at 26-33 DEGC under an illumination condition after inoculation. According to the invention, malodorous pollutants in garbage are decomposed and degraded through beneficial flora activity and fermentation activeproducts, not only waste stink of pig farms is eliminated, but also harmful pathogenic bacteria can be inhibited and sterilized, and meanwhile, volatile stink and pathogenic bacteria can be effectively controlled by spraying air above and around a waste field of the pig farms.

Description

technical field [0001] The invention relates to the technical field of deodorizing and anti-epidemic microbial liquid, in particular to a method for preparing a compound microbial liquid with deodorizing and anti-epidemic functions. Background technique [0002] Pigs have a long history of raising pigs in China. They are the breed of livestock and poultry with the widest range of breeding and involving the most farmers. They occupy an important position in the structure of animal husbandry in China. Sewage, feces, stench, etc. cause serious environmental pollution. Especially in recent years, with the rapid development of my country's aquaculture industry, the problem of aquaculture waste pollution has become increasingly prominent. It has become the focus and hot issue of complaints from the public, and the pressure on environmental protection is increasing It also brings hidden dangers to the prevention and control of animal diseases and the quality and safety of farmed prod...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/16C12N1/20B01D53/84C12R1/225C12R1/02C12R1/125
CPCC12N1/36C12N1/16C12N1/20B01D53/84Y02A50/20
Inventor 安德荣陈国参张英涛岳丹丹安航许博雄
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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