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New material containing tbFGF and use thereof

A tbfgf-peg-ve, PEG-VE technology, applied in the field of new materials containing tbFGF, can solve the problems of unsatisfactory sustained release effect, large liposome particle size, poor stability, etc. Absorption and utilization, enhanced sustained release, reduced multidrug resistance

Inactive Publication Date: 2020-01-03
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compared with free drugs, liposome-encapsulated drugs can play a certain sustained-release effect, but the sustained-release effect is still unsatisfactory, and the particle size of liposomes is large, not easily absorbed, and the stability is not good. Good, needs further improvement

Method used

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  • New material containing tbFGF and use thereof
  • New material containing tbFGF and use thereof
  • New material containing tbFGF and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The structure of embodiment 1 liposome carrier of the present invention (mPEG 2000 -VE with tbFGF-PEG 2000 Preparation and characterization of -VE)

[0077] 1 Experimental materials

[0078] 1.1 Instrument

[0079] Constant temperature heating magnetic stirrer: DF-101S, Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.;

[0080] Circulating water multi-purpose vacuum pump: SHB-III, Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.;

[0081] Rotary evaporator: Rotavapor: RII, Switzerland BUCHI;

[0082] Analytical balance: AUW120D, d=0.1mg / 0.01mg, Shimadzu, Japan;

[0083] Three-purpose ultraviolet instrument: IF-I type, Shanghai Gucun Electro-optical Instrument Factory;

[0084] Ultrasonic breaker: VCX-500, ultrasonic probe: 6mm, SONICS company;

[0085] NMR spectrometer AV-11, 400MHz Swiss Bruker;

[0086] Vacuum drying oven: VT6130M type, Heraeus, Germany;

[0087] Fourier infrared spectrometer: Nicolet 6700 Thermoelectric Corporation of...

Embodiment 2

[0170] Example 2 Preparation of drug-containing liposome carrier of the present invention and its effect verification construction

[0171] 1 Experimental materials

[0172] 1.1 Instrument

[0173] Rotary evaporator: Rotavapor.RII, Switzerland BUCHI;

[0174] Circulating water type multi-purpose vacuum pump: SHB-IIIS, Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.;

[0175] High pressure homogenizer: EmulsiFlex-05, American AVESTIN;

[0176] Analytical balance: AUW120D, d=0.1mg / 0.01mg, Shimadzu, Japan;

[0177] Constant temperature heating magnetic stirrer: DF-101S, Gongyi City Yuhua Instrument Co., Ltd.;

[0178] Vacuum drying oven: ZKD-4025A, Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.;

[0179] Pure water meter: Milli-Q, Millipore, USA;

[0180] Refrigerator: BCD539WH, Haier;

[0181] Freeze dryer: Beijing Boyikang Experimental Instrument Co., Ltd.;

[0182]Vortex mixer: HQ-60-Ⅱ, the same as in the north;

[0183] Nano particle siz...

Embodiment 3

[0289] Example 3 In vitro cytotoxicity and cellular uptake experiments of the drug-containing liposome carrier of the present invention

[0290] Get the DTX-tbFGF-LPs, DTX-PEG-LPs, and DTX-LPs prepared in Example 2, and use Free DTX as a control to carry out the following experiments:

[0291] 1 Experimental materials

[0292] 1.1 Instrument

[0293] Analytical balance: AUW120D d=0.1mg / 0.01mg, Shimadzu, Japan;

[0294] Constant temperature heating magnetic stirrer: DF-101S Gongyi City Yuhua Instrument Co., Ltd.;

[0295] Pure water meter: Milli-Q, Millipore, USA;

[0296] Multifunctional microplate reader: SpectraMax M5, Molecular Devices, USA;

[0297] Biological safety cabinet: Class II Biological Safety Cabinet, ESCO;

[0298] Inverted microscope: CKX31, Japan Olympus;

[0299] Centrifuge: SORVALL ST16, Thermo Scientific, USA;

[0300] Constant temperature incubator: Cell Culture CO 2 Incubator, ESCO;

[0301] Autoclave: Labo Auto clave MLS-378, SANYO;

[0302] Vort...

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Abstract

The invention discloses materials PEG-VE and tbFGF-PEG-VE. A structural formula of tbFGF-PEG-VE is as shown in a formula (II) in the specification, wherein a substituent group represents a polypeptidein the specification, and n is 1000-4000. The invention also provides a use of the materials in preparation of liposomes, and the prepared liposomes. PEG-VE and tbFGF-PEG-VE are adopted to modify liposomes, so that the performance of the liposomes is significantly improved, and the clinical application prospect is good.

Description

technical field [0001] The present invention relates to a new material containing tbFGF and its use. Background technique [0002] Many antineoplastic drugs present many shortcomings in clinical application, including poor therapeutic effect, dose-limiting toxicity, and poor patient tolerance, among which the poor drug distribution leads to a large toxic effect on normal tissues, which promotes the development of new targets. Development towards drug delivery formulations. Recently, targeted drug delivery systems based on nanotechnology have developed rapidly. For example, micelles, liposomes, and solid lipid nanoparticles are widely used in cancer chemotherapy to improve drug solubility, prolong half-life in vivo, and increase drug delivery in tumor tissues. And the distribution in cells, so as to achieve the best anti-tumor effect and the smallest toxic side effects. [0003] Liposomes (Liposomes) are closed vesicles (hollow) with a bilayer structure made of phospholipid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G65/332A61K47/22A61K47/42A61K9/127A61P35/00A61K31/337
CPCA61K9/1271A61K9/1277A61K31/337A61K47/22A61K47/42A61P35/00C08G65/3328
Inventor 陈俐娟温姣琳
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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