Method for generating liquid droplet array on microfluidic chip

A technology of microfluidic chips and microwell arrays, applied in biochemical equipment and methods, measurement/testing of microorganisms, fluid controllers, etc., can solve problems such as cross-contamination of micropores, high price and complexity of surfactants, etc. Achieve the effect of good physical isolation, convenient assembly and large tolerance
CN110643483AActive Publication Date: 2020-01-03SHANGHAI JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAOTONG UNIV
Publication Date
2020-01-03

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Abstract

The invention discloses a method for generating a liquid droplet array on a microfluidic chip, and relates to the field of liquid droplet generation. The method for generating the liquid droplet arrayon the microfluidic chip includes the following steps that an upper chip and a lower chip are assembled to an initial position, and a fluid pipeline of the upper chip partially or completely covers amicrowell array of the lower chip; a solution is injected into the chip, and the solution partially or completely fills the microwell array of the lower chip; the upper chip and the lower chip are relatively moved to the liquid dividing position, the fluid pipeline of the upper chip and the microwell array of the lower chip no longer overlap, and the solution is dispersed in the microwell array to form the liquid droplet array. The contact surface between the upper chip and the lower chip is hydrophobic, and the microwell array fully physically isolates the generated liquid droplets to avoidcross-contamination. According to the method for generating the liquid droplet array on the microfluidic chip, the device and operation of liquid droplet generation are simplified, the design is flexible, and the size and shape of the generated liquid droplets can be effectively controlled.
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Description

technical field

[0001] The invention relates to the field of droplet generation, in particular to a method for generating a droplet array on a microfluidic chip. Background technique

[0002] Droplets have a wide range of applications in physics, chemistry, biology, and medicine. Among them, a large number (usually more than 100) droplet arrays have shown unique advantages in gene, protein and cell analysis.

[0003] Among them, digital PCR gene amplification is a technology based on a large number of independent micro-droplets to accurately quantify the target gene. By dispersing the reaction solution into picoliter or nanoliter microdroplets or reaction microwells, each microdroplet or microwell contains at most one copy of the target gene. For example, some microdroplets or microwells contain a gene of interest and other microdroplets or microwells do not contain the gene of interest. Through the specific amplification of the target gene in the micro-droplet or micro-w...

Claims

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