Method for detecting cytokines secreted by specific T cells in lung cancer or intestinal cancer

Abstract

The invention belongs to the technical field of medical detection application and discloses a method for detecting cytokines secreted by specific T cells in lung cancer or intestinal cancer. A systemis composed of a detection sample, a target, a stimulation antigens and / or positive control antigens of the detection sample and conventional reagents of the detection sample. The sample in the detection system is mononuclear cells after peripheral blood of a lung cancer or intestinal cancer patient is separated, and cytokines secreted by the specific T cells are detected through a plurality of rounds of stimulation of polypeptide. According to the method, the mononuclear cells are induced into DC cells; the DC cells and the T cells are co-cultured; after the polypeptide is added and three rounds of stimulation is carried out, the cytokines secreted by the specific T cells are detected; and under the condition that detection sensitivity is not influenced, detection time of the whole systemis reduced. According to the detection system provided by the invention, an application method is simple, rapid and efficient. The detection time is reduced. Detection efficiency is improved. The detection system provided by the invention is wide in application range and has huge market value.

Description

technical field [0001] The invention belongs to the technical field of medical detection application, and in particular relates to a method for detecting cytokines secreted by specific T cells in lung cancer or intestinal cancer. Background technique [0002] Currently, the closest prior art: [0003] Cytokine (CK) is stimulated by immune cells (such as monocytes, macrophages, T cells, B cells, NK cells, etc.) and certain non-immune cells (endothelial cells, epidermal cells, fibroblasts, etc.) A class of small molecular proteins that are synthesized and secreted with a wide range of biological activities. It has multiple functions such as regulating innate immunity and adaptive immunity, hematopoiesis, cell growth and repair of damaged tissues. Cytokines can be classified into interleukins, interferons, tumor necrosis factor superfamily, colony-stimulating factors, chemokines, growth factors, and the like. Many cytokines play a role in the body through paracrine, autocrin...

AI Technical Summary

Problems solved by technology

[0008] (1) The diagnosis of tuberculosis commonly used in clinical practice mainly relies on clinical symptoms, impact diagnosis and etiology diagnosis, and is not sensitive to the diagnosis of latent infection of Mycobacterium tuberculosis

[0009] (2) In the process of tuberculosis screening, the sensitivity and specificity of direct detection of pathogens or detection of Mycobacterium tuberculosis antibodies are not ideal

[0010] (3) In vitro detection of T cells has the defect that the detection time is too long

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