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A kind of sesame peptide powder and its preparation method and application

A technology for sesame peptides and sesame seeds, applied in the field of preparation and enzymolysis, can solve the problems of harsh preparation conditions of sesame peptides, high cost of gel chromatography columns, unsuitable for large-scale production, etc., and achieves improved pulmonary function, low cost, Add less effect

Active Publication Date: 2021-12-03
SINPHAR TIAN LI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, n-hexane is used as a degreasing solvent, and n-hexane is a flammable and explosive solvent, which brings safety hazards to industrial production, and n-hexane will remain in the product, causing harm to the human body. The protein determination method detects sesame peptides, so the peptide content is inaccurate and unscientific
Wang Zhenbin and others used ultrasonic and microwave technology to assist enzymatic hydrolysis to obtain sesame peptides. Ultrasonic and microwave technologies are not suitable for large-scale production, resulting in limitations in the use of the method
Li Jia et al. used alkaline protease to hydrolyze the sesame protein, then used gel chromatography to separate and purify to obtain antioxidant peptides. The gel chromatography column was expensive and the post-processing was complicated, which made it difficult to produce on a large scale.
It can be seen that the preparation conditions of the above sesame peptides are harsh, the process steps are cumbersome, and it is difficult to achieve large-scale production. Therefore, finding a set of preparation methods for sesame peptides with simple process, low cost, high content, and high activity has become a search for large-scale production. One of the research hotspots

Method used

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  • A kind of sesame peptide powder and its preparation method and application
  • A kind of sesame peptide powder and its preparation method and application
  • A kind of sesame peptide powder and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0046] Deoil the black sesame by cold pressing to obtain defatted sesame meal, weigh 100kg of sesame meal, record it as A, add 1000L of water, adjust the pH to 8.0-8.5, and extract at room temperature for 1 hour; Pour in an equal amount of sesame meal, record it as B, adjust the pH to 8.0-8.5, and extract at room temperature for 1 hour; after B completes the first extraction, the filtrate is set aside, and the filter residue continues for the second extraction; after A completes the second extraction, Discard the filter residue, pour the filtrate into the same amount of sesame meal, record it as C, adjust the pH to 8.0-8.5 and extract at room temperature for 1 hour; after B completes the second extraction, discard the filter residue, pour the filtrate into C to complete the filter residue of the first extraction Extract for 1 hour, and the filtrate from the first extraction of C is set aside; after the second extraction of sample C, the filter residue is discarded, and the filt...

preparation Embodiment 2

[0048] Deoil the black sesame by cold pressing to obtain defatted sesame meal, weigh 100kg of sesame meal, record it as A, add 500L of water, adjust the pH to 9.0-9.5, and extract at room temperature for 1 hour; Pour in an equal amount of sesame meal, record it as B, adjust the pH to 9.0-9.5, and extract at room temperature for 1 hour; after B completes the first extraction, the filtrate is set aside, and the filter residue continues for the second extraction; after A completes the second extraction, Discard the filter residue, pour the filtrate into the same amount of sesame meal, record it as C, adjust the pH to 9.0-9.5 and extract at room temperature for 1 hour; after B completes the second extraction, discard the filter residue, pour the filtrate into C to complete the filter residue of the first extraction and the filtrate from the first extraction of C was set aside; after the second extraction of sample C, the filter residue was discarded, and the filtrate was set aside;...

preparation Embodiment 3

[0050] Deoil the black sesame by cold pressing to obtain defatted sesame meal, weigh 100kg of sesame meal, record it as A, add 500L of water, adjust the pH to 8.5-9.0, and extract at room temperature for 0.5h; after the extraction is completed, filter and filter the residue for secondary extraction. Pour the filtrate into an equal amount of sesame meal, record it as B, adjust the pH to 8.5-9.0, and extract at room temperature for 0.5h; after B completes the first extraction, the filtrate is set aside, and the filter residue continues for the second extraction; A completes the second extraction Finally, discard the filter residue, pour the filtrate into the same amount of sesame meal, record it as C, adjust the pH to 8.5-9.0 and extract at room temperature for 0.5h; after B completes the second extraction, discard the filter residue, pour the filtrate into C to complete the first extraction The extracted filter residue was extracted for 0.5h, and the filtrate from the first extr...

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Abstract

The invention relates to a sesame peptide powder and its preparation method and application. The peptide content of the sesame peptide powder is more than 90 wt%, and the molecular weight of more than 90% of the sesame peptide is less than 1000 Dalton. The defatted sesame meal is subjected to protein extraction, enzymatic hydrolysis, purification, concentration and drying to obtain sesame peptide. This sesame peptide powder has good free radical scavenging activity, improves sleep quality, enhances human immunity, and has the effect of anti-smog at the same time. The method of the invention is easy to operate, high in yield and low in cost, stable in product quality, environmentally friendly in technology and suitable for large-scale production.

Description

technical field [0001] The invention relates to a high-purity, low-molecular-weight sesame peptide product, the peptide content of which is more than 90 wt%, and the molecular weight is less than 1000Dalton and more than 90%. The invention also relates to the preparation and enzymatic hydrolysis of the sesame protein to generate the oligomeric sesame peptide powder. The peptide powder can be used as food, health food or medicine. Background technique [0002] Sesamum (Sesamum indicm L.) belongs to the family Flaxaceae, and is the seed of flax, which is distributed in tropical regions and some temperate regions. Sesame contains a large amount of fat and protein, and is rich in sterols, sesamin, sesamol, lecithin compounds, dietary fiber, sugars, vitamins and minerals. Sesame can be divided into four categories according to color, namely white sesame, black sesame, yellow and white sesame, and variegated sesame, among which black sesame has the best health care effect. Tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K4/10C07K1/34C07K1/14C12P21/06A61K38/01A61K8/64A61P25/20A61P37/04A61P11/00A61P1/00A23L33/18
CPCA23V2002/00A61K8/645A61K38/00A61K2800/10A61K2800/805A23L33/18A61P1/00A61P11/00A61P25/20A61P37/04C07K4/10C12P21/06A23V2200/314A23V2250/55Y02P20/54
Inventor 王昭日刘明川杨胜杰
Owner SINPHAR TIAN LI PHARMA