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A bisporus ethylene receptor protein

A technology for ethylene and receptor proteins of Agaricus bisporus, which is applied in the direction of using vectors to introduce foreign genetic material, peptides, chemical instruments and methods, etc.

Active Publication Date: 2022-06-14
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the related research is still relatively limited, it is not clear whether there is an ethylene signal transduction pathway similar to that in higher plants in Agaricus bisporus

Method used

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  • A bisporus ethylene receptor protein
  • A bisporus ethylene receptor protein
  • A bisporus ethylene receptor protein

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Embodiment 1

[0031] It should be noted that in the previous research on the Agaricus bisporus genome, it was found that there are several hybrid histidine kinases in the Agaricus bisporus genome, and the Ab201390 protein has all domain structures similar to plant ethylene receptors, including: 5 spanning The membrane region contains a cysteine ​​residue (Cys130) in the third transmembrane region; and the second transmembrane region of the existing Arabidopsis ethylene receptor ETR1 also contains a cysteine ​​residue (Cys65 ) (this residue is an amino acid necessary for ETR1 to bind ethylene). Based on these structural comparison results, the inventor speculated that Ab201390 is the ethylene receptor protein (AbEBD1) in Agaricus bisporus, which can bind ethylene. Based on this hypothesis, the inventors cloned and verified the actual function of the protein by using fluorescent expression technology. The specific experimental verification process is briefly introduced as follows.

[0032] ...

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Abstract

The application belongs to the field of plant genome technology, and in particular relates to an vinyl receptor in the Agaricus bisporus genome. The application belongs to the field of plant genome technology, and in particular relates to an vinyl receptor in the Agaricus bisporus genome. Based on the results of structural comparison, the inventor speculated that Ab201390 is the ethylene receptor protein (AbEBD1) in Agaricus bisporus, which can bind ethylene. After further practical verification, it was found that the protein has ethylene binding ability, but its binding ability is significantly weaker than that of the Arabidopsis ethylene receptor ETR1. However, based on this research result, through genetic engineering technology, it can still lay a certain technical foundation for the cultivation of new varieties of Agaricus bisporus, or technical improvements such as Agaricus bisporus preservation.

Description

technical field [0001] The application belongs to the field of plant genome technology, and in particular relates to an ethylene receptor protein in the genome of Agaricus bisporus. Background technique [0002] Agaricus bisporus is the edible fungus with the widest cultivation range and the largest output and consumption in the world, and it is also the edible fungus with the largest export volume in my country. Physiological studies have shown that Agaricus bisporus is similar to higher plants in that they can synthesize ethylene, and the synthesized ethylene can further inhibit the growth of mycelium and the formation of fruiting bodies, and accelerate the maturation and aging of postharvest fruiting bodies. However, because the relevant research is still relatively limited, it is not clear whether there is an ethylene signal transduction pathway similar to that in higher plants in Agaricus bisporus. [0003] Studies have shown that in higher plants, ethylene receptors a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/375C12N15/10C12N15/80
CPCC07K14/375C12N15/80
Inventor 邱立友郜熙阳张君高玉千李亚楠张朝辉李涛田芳
Owner HENAN AGRICULTURAL UNIVERSITY