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Agaricus bisporus ethylene protein receptor

A technology of Agaricus bisporus ethylene and Agaricus bisporus, applied in the direction of introducing foreign genetic material, peptides, peptides, etc. by using vectors, which can solve the problems of limited and unclear research

Active Publication Date: 2020-01-10
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the related research is still relatively limited, it is not clear whether there is an ethylene signal transduction pathway similar to that in higher plants in Agaricus bisporus

Method used

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  • Agaricus bisporus ethylene protein receptor
  • Agaricus bisporus ethylene protein receptor
  • Agaricus bisporus ethylene protein receptor

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Experimental program
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Embodiment 1

[0031] It should be noted that in the previous research on the Agaricus bisporus genome, it was found that there are several hybrid histidine kinases in the Agaricus bisporus genome, and the Ab201390 protein has all domain structures similar to plant ethylene receptors, including: 5 spanning The membrane region contains a cysteine ​​residue (Cys130) in the third transmembrane region; and the second transmembrane region of the existing Arabidopsis ethylene receptor ETR1 also contains a cysteine ​​residue (Cys65 ) (this residue is an amino acid necessary for ETR1 to bind ethylene). Based on these structural comparison results, the inventor speculated that Ab201390 is the ethylene receptor protein (AbEBD1) in Agaricus bisporus, which can bind ethylene. Based on this hypothesis, the inventors cloned and verified the actual function of the protein by using fluorescent expression technology. The specific experimental verification process is briefly introduced as follows.

[0032] ...

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Abstract

The present application belongs to the technical field of plant genomes and particularly relates to an ethylene protein receptor in an agaricus bisporus genome. Based on structural comparison results,Ab201390 is speculated to be an ethylene receptor protein (AbEDB1) in agaricus bisporus and can bind to ethylene. After further actual verification, the protein has an ethylene binding function and has binding capacity significantly weaker than that of an arabidopsis thaliana ethylene receptor ETR1. However, based on results of researches and through genetic engineering techniques, the agaricus bisporus ethylene protein receptor can still lay a certain technical foundation for culture of agaricus bisporus new varieties or preservation of agaricus bisporus, etc.

Description

technical field [0001] The application belongs to the field of plant genome technology, and in particular relates to an vinyl receptor in the genome of Agaricus bisporus. Background technique [0002] Agaricus bisporus is the edible fungus with the widest cultivation range and the largest output and consumption in the world, and it is also the edible fungus with the largest export volume in my country. Physiological studies have shown that Agaricus bisporus is similar to higher plants in that they can synthesize ethylene, and the synthesized ethylene can further inhibit the growth of mycelium and the formation of fruiting bodies, and accelerate the maturation and aging of postharvest fruiting bodies. However, because the relevant research is still relatively limited, it is not clear whether there is an ethylene signal transduction pathway similar to that in higher plants in Agaricus bisporus. [0003] Studies have shown that in higher plants, ethylene receptors are an impor...

Claims

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Application Information

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IPC IPC(8): C07K14/375C12N15/10C12N15/80
CPCC07K14/375C12N15/80
Inventor 邱立友郜熙阳张君高玉千李亚楠张朝辉李涛田芳
Owner HENAN AGRICULTURAL UNIVERSITY