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A bisporus ethylene receptor protein ab143539

A technology for ethylene and receptor protein of Agaricus bisporus, which is used in DNA preparation, recombinant DNA technology, peptides, etc.

Active Publication Date: 2022-06-14
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the related research is still relatively limited, it is not clear whether there is an ethylene signal transduction pathway similar to that in higher plants in Agaricus bisporus

Method used

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  • A bisporus ethylene receptor protein ab143539
  • A bisporus ethylene receptor protein ab143539
  • A bisporus ethylene receptor protein ab143539

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Embodiment 1

[0031] It should be noted that in the previous research on the Agaricus bisporus genome, it was found that there are several hybrid histidine kinases in the Agaricus bisporus genome, and the Ab143539 protein has all domain structures similar to plant ethylene receptors, including: 5 spanning Membrane region, as well as histidine kinase domain and receptor effector regulator, but no GAF domain, but contains a cysteine ​​residue (Cys291) in the third transmembrane region; while the existing Arabidopsis ethylene receptor The second transmembrane domain of ETR1 also contains a cysteine ​​residue (Cys65) (this residue is an amino acid necessary for ETR1 to bind ethylene). Based on these structural comparison results, the inventor speculated that Ab143539 is the ethylene receptor protein (AbEBD2) in Agaricus bisporus, which can bind ethylene. Based on this hypothesis, the inventors cloned and verified the actual function of the protein by using fluorescent expression technology. Th...

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Abstract

The application belongs to the field of plant genome technology, and in particular relates to the vinyl protein receptor Ab143539 in the Agaricus bisporus genome. There are five transmembrane regions in its structure, and a cysteine ​​residue is contained in the third transmembrane region. The specific base sequence is shown in SEQ ID NO.1. Based on the results of structural comparison, the inventor speculated that Ab143539 is the ethylene receptor protein (AbEBD2) in Agaricus bisporus, which can bind ethylene. After further practical verification, it was found that the protein has ethylene binding ability, but its binding ability is significantly weaker than that of the Arabidopsis ethylene receptor ETR1. However, based on this research result, through genetic engineering technology, it can still lay a certain technical foundation for the cultivation of new varieties of Agaricus bisporus, or technical improvements such as Agaricus bisporus preservation.

Description

technical field [0001] The application belongs to the field of plant genome technology, and in particular relates to an ethylene receptor protein Ab143539 in the genome of Agaricus bisporus. Background technique [0002] Agaricus bisporus is the edible fungus with the widest cultivation range and the largest output and consumption in the world, and it is also the edible fungus with the largest export volume in my country. Physiological studies have shown that Agaricus bisporus is similar to higher plants in that they can synthesize ethylene, and the synthesized ethylene can further inhibit the growth of mycelium and the formation of fruiting bodies, and accelerate the maturation and aging of postharvest fruiting bodies. However, because the relevant research is still relatively limited, it is not clear whether there is an ethylene signal transduction pathway similar to that in higher plants in Agaricus bisporus. [0003] Studies have shown that in higher plants, ethylene re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/375C12N15/31C12N15/10A01H15/00
CPCC07K14/375C12N15/8261
Inventor 邱立友郜熙阳高玉千李亚楠张君李涛张朝辉田芳
Owner HENAN AGRICULTURAL UNIVERSITY
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