Penaeus monodon glycogen synthase kinase GSK3beta gene and application thereof

Abstract

The invention discloses a penaeus monodon glycogen synthase kinase GSK3beta gene. An alkali base sequence of the penaeus monodon glycogen synthase kinase GSK3beta gene is as shown in SEQ ID NO: 1. Theinvention further discloses a coding protein of the penaeus monodon glycogen synthase kinase GSK3beta gene. An amino acid sequence of the coding protein is as shown in SEQ ID NO: 2. The invention further discloses application of the penaeus monodon glycogen synthase kinase GSK3beta gene or the coding protein in improving ammonia-nitrogen tolerance of penaeus monodon and SNP loci related to ammonia-nitrogen tolerance of the penaeus monodon. The SNP locus is the 940 locus of the alkali base sequence as shown in the SEQ ID NO: 1, and an alkali base mutates into G from C. The screened and identified 940 locus of the penaeus monodon glycogen synthase kinase GSK3beta gene is the SNP locus related to ammonia-nitrogen tolerance of the penaeus monodon, and important reference marks are provided for selecting and breeding molecules of new species of ammonia-nitrogen tolerance of the penaeus monodon.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a glycogen synthase kinase GSK3β gene of Penaeus monodon and its application. Background technique [0002] Glycogen synthase kinase GSK3 (Glycogen synthase kinase-3) is a multifunctional serine / threonine protein kinase with two highly conserved isoforms GSK3α and GSK3β. In the late 1970s, GSK was discovered and identified, and its function was initially considered to be the phosphorylation of liver glycogen synthase to regulate the activity of the enzyme in catalyzing glycolysis. GSK3 has the hallmarks of an unconventional constitutively active kinase characterized by a substrate that often requires prephosphorylation by another kinase, and GSK3 responds on the two major pathways known to affect it (insulin and Wnt pathways) The way of stimulation is inhibition rather than activation, which is the main feature that distinguishes GSK3 from other kinases [71...

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Problems solved by technology

However, in crustaceans, research reports on the GSK3β gene are still lacking.

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