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A plastic method for copepod samples before identification

A technology of copepods and samples, which is applied in the field of pre-identification of copepod samples, can solve the problems of unfavorable observation and achieve the effect of convenient observation

Active Publication Date: 2021-06-18
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcoming that copepods are unfavorable to observation after long-term storage in order to overcome the shortcomings of the prior art, and propose a plastic method for copepod samples before identification, which can restore the curled up copepods , for easy observation

Method used

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  • A plastic method for copepod samples before identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for shaping a copepod sample before identification, the steps are as follows:

[0018] Step a: Put the copepods to be shaped into a neutral formalin solution and store in a cool and dry place; the storage time is 30 days; the raw materials of the neutral formalin solution are 100ml formaldehyde, 900ml distilled water, 4g Sodium dihydrogen phosphate, 6.5 grams of sodium monohydrogen phosphate.

[0019] Step b: filter with a 90-mesh sieve; filter to obtain copepods;

[0020] Step c: Put the filtered copepods back into a new neutral formalin solution, and put the neutral formalin solution into the copepods on a shaker at 40°C, The shaker was oscillated at 60r / min for 50 minutes; water molecules entered the gaps in the tissues of the copepods, thereby restoring the shape of the copepods.

[0021] Copepods, such as Daphnia, are stored in formalin solution for a long time, such as one year, the formaldehyde in formalin will be oxidized to formic acid, and the body ...

Embodiment 2

[0023] Such as figure 1 As shown, the general method of this embodiment is consistent with that of Embodiment 1, the difference is that in step b, a filter device is used to filter, and the filter device includes a base 3, a filter cup 1 is fixed above the base 3, and the inside of the filter cup 1 A sieve 2 is provided at the bottom, and a guide tube 4 is connected to the bottom of the filter cup 1. A pillar 5 is fixedly connected to the base 3, and a slider 6 is slidably connected to the pillar 5. The guide tube 4 is far away from the One end of the filter cup 1 is fixedly connected to the slider 6, and a water cup 7 is provided below the end of the guide tube 4 away from the filter cup 1. The inner diameter of the water cup 7 is consistent with the inner diameter of the filter cup 1. The inner diameter of the water cup 7 A floating ball 9 is provided, and the support 5 is rotatably connected with a guide wheel 8 above the slider 6, and a bracket 11 is fixedly connected to t...

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Abstract

The invention discloses a method for plasticizing copepod samples before identification, and relates to the technical field of experimental methods for copepods; the steps are as follows: step a: put the copepods to be plasticized into a neutral formalin solution, Store in a cool and dry place; step b: filter to obtain copepods; step c: put the filtered copepods back into a new neutral formalin solution, so that water molecules can enter the gaps in the copepod body tissues inside, so that the copepod's body is restored. The invention can restore the posture of the curled-up copepod and is convenient for observation.

Description

technical field [0001] The invention belongs to the technical field of experimental methods of copepods, and in particular relates to a shaping method for copepod samples before identification. Background technique [0002] Copepods belong to the phylum Arthropoda, Crustacea, and Copepoda. There are 7,500 species of copepods in the world, of which about 4,500 species of marine copepods have been identified. Due to the large number, wide distribution, short growth cycle and small size of copepods, species identification is difficult. As the country vigorously promotes the construction of water ecological civilization and objectively evaluates the water quality of water bodies, the identification of copepods is particularly important. After long-term storage in fixative solution, the appendages and antennae of copepods curl up, which is not conducive to observation, and a pretreatment and recovery method is urgently needed. Contents of the invention [0003] The purpose of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/59A01N1/00
CPCA01N1/00A01K61/59Y02A40/81
Inventor 周超金扬湖
Owner ZHEJIANG OCEAN UNIV