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Kit for detecting PD-L1 expression level

A PD-L1 and expression level technology, applied in recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of high detection limit and inability to provide absolute quantification, so as to reduce false positive and convenient Auxiliary diagnosis and clinical treatment guidance, high accuracy effect

Inactive Publication Date: 2020-01-17
宁波胤瑞生物医学仪器有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, on the one hand, this detection method cannot provide absolute quantification, and on the other hand, the detection limit is relatively high.

Method used

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  • Kit for detecting PD-L1 expression level
  • Kit for detecting PD-L1 expression level
  • Kit for detecting PD-L1 expression level

Examples

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Embodiment 1

[0052] A kit for detecting the expression level of PD-L1, the kit comprising PD-L1 specific primers, GAPDH specific primers, PD-L1 probes, and GAPDH probes;

[0053] The PD-L1-specific primers include the following nucleotide sequences

[0054] Upstream primer: 5-CCGCTGCATGATCAGCTATGG-3SEQ ID NO: 1;

[0055] Downstream primer: 5-GAGGTGACTGGATCCACAACC-3SEQ ID NO: 2;

[0056] The PD-L1 probe includes the following nucleotide sequence

[0057] 5-CAAGGACCTATATGTGGTAG-3 SEQ ID NO: 3;

[0058] The GAPDH-specific primers include the following nucleotide sequences

[0059] Upstream primer: 5-CTCTGCTCCTCCTGTTCGAC-3SEQ ID NO: 4;

[0060] Downstream primer: 5-ATGGTGTCTGAGCGATGTGG-3SEQ ID NO: 5;

[0061] The GAPDH probe includes the following nucleotide sequence

[0062] 5-CCTGGCCAAGGTCATCCATGACAACTT-3 SEQ ID NO:6.

[0063] The method for detecting the expression level of PD-L1 using the above kit comprises the following steps:

[0064] S1 For the selected PD-L1 and GAPDH sequences,...

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Abstract

The invention belongs to the technical field of PD-L1 expression detection, and particularly relates to a kit for detecting PD-L1 expression level. The kit comprises a PD-L1 specific primer, a GAPDH specific primer, a PD-L1 probe and a GAPDH probe. Compared with the prior art, the kit based on a digital PCR detection system has the advantages that full automatic detection is achieved after sampling, manual operation interference is reduced, results are stable, accuracy is high, the data amplification effect is good, and the kit can be conveniently used for auxiliary diagnosis and clinical treatment guidance.

Description

technical field [0001] The invention belongs to the technical field of PD-L1 expression detection, and in particular relates to a kit for detecting the expression level of PD-L1. Background technique [0002] With the official approval of the China Food and Drug Administration (CFDA) for the launch of the first PD-1 inhibitor nivolumab in China, it indicates that my country has entered a new era of immunotherapy. Tumor immunotherapy is to mobilize the body's own immune system and enhance anti-tumor immunity, thereby inhibiting and killing tumor cells. Its appearance has changed the pattern of traditional surgical treatment, radiotherapy and chemotherapy, and is the most promising in the current field of tumor treatment. one of the research directions. [0003] In the interaction between tumor cells and immune effector cells, the ligand of programmed cell death-ligand 1 (PD-L1) on the surface of tumor cells and the programmed death on the surface of immune effector cells (T ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q2531/113C12Q2521/107C12Q2527/127C12Q2545/101C12Q2563/107
Inventor 金鑫浩刘一博隋硕任鲁风张未来俞育德于军
Owner 宁波胤瑞生物医学仪器有限责任公司