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Application of MMP7, CTSE or LAMC2 protein in preparation of diagnosis reagent for intrahepatic cholangiocarcinoma

A technique for intra-cholangiocarcinoma and cell carcinoma, which is applied in the field of medical bioengineering and can solve the problems of unsatisfactory sensitivity and specificity.

Inactive Publication Date: 2020-01-31
杭州道合天孚生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

Although carbohydrate antigen 125, carbohydrate antigen 19-9, and carcinoembryonic antigen are clinically used as reference markers for the diagnosis of cholangiocarcinoma, they all have high expression values ​​in ductal cancers, and their sensitivity and The specificity is not ideal

Method used

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  • Application of MMP7, CTSE or LAMC2 protein in preparation of diagnosis reagent for intrahepatic cholangiocarcinoma
  • Application of MMP7, CTSE or LAMC2 protein in preparation of diagnosis reagent for intrahepatic cholangiocarcinoma
  • Application of MMP7, CTSE or LAMC2 protein in preparation of diagnosis reagent for intrahepatic cholangiocarcinoma

Examples

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Embodiment 1

[0038] Example 1. A hospital selected 98 volunteers, including 39 patients with intrahepatic cholangiocarcinoma, 22 healthy people, 20 patients with benign biliary tract disease and 17 patients with hepatocellular carcinoma. Extract biological samples from volunteers, record characteristic parameters, and use kits for specific detection. The characteristic parameters of the test group are shown in Table 1.

[0039] Table 1 presents the clinical characteristics of 39 patients with intrahepatic cholangiocarcinoma, 17 patients with hepatocellular carcinoma, and 20 patients with benign biliary tract disease.

[0040]

[0041]

[0042] (In the table: a Fisher's exact test; b chi-square test; c variance test)

[0043] Use the commercially available antibody sandwich method to detect the three proteins, add the sample to the antibody-coated microwell, incubate at 37°C for 1 hour, add biotinylated antibody (diluted 100 times), incubate again at 37°C for 1 hour, wash Add the dete...

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Abstract

The invention relates to the technical field of medical bioengineering, and aims to provide application of MMP7, CTSE or LAMC2 protein in preparation of a diagnosis reagent for intrahepatic cholangiocarcinoma. At least one protein is used for preparing a reagent capable of identifying and diagnosing intrahepatic cholangiocarcinoma and distinguishing primary hepatocellular carcinoma, namely, the three proteins are used independently or in combination to identify and distinguish hepatocellular carcinoma and intrahepatic cholangiocarcinoma. The invention provides a reagent or kit product preparedfrom the three proteins, and the expression level of the protein in blood is detected by adopting an immunological related method to serve as a basis for diagnosing an intrahepatic cholangiocarcinomamarker. The possibility of judging intrahepatic cholangiocarcinoma, benign biliary tract diseases and hepatocellular carcinoma for a subject, and the possibility of judging the health state for the subject can be accurately and specifically provided for the subject, and a basis is provided for a next diagnosis and treatment scheme of a doctor.

Description

technical field [0001] The invention belongs to the technical field of medical bioengineering, and particularly relates to the application of MMP7, CTSE or LAMC2 protein in the preparation of diagnostic reagents for intrahepatic cholangiocarcinoma. Background technique [0002] Intrahepatic cholangiocarcinoma accounts for 10%-15% of primary liver tumors and is the second most common primary liver cancer. Intrahepatic cholangiocarcinoma has no obvious clinical symptoms in the early stage, and most patients have developed to an advanced stage when they are diagnosed, and have lost the opportunity for surgery. Intrahepatic cholangiocarcinoma is not sensitive to chemotherapy, immunotherapy, and radiotherapy, and the more effective treatment is surgical resection. The overall survival of patients with intrahepatic cholangiocarcinoma after diagnosis is generally 6-12 months, and the 5-year survival rate is less than 5%. The 5-year survival rate of patients after resection is only...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/573
CPCG01N33/573G01N33/57407G01N33/57484G01N33/68G01N2333/78G01N2333/96472G01N2333/96494
Inventor 姬峻芳张剑娟
Owner 杭州道合天孚生物科技有限公司
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